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F1903 Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified

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F1903
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      CULT
      Description
      Catalogue NumberF1903
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionFibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified
      OverviewHeparin-binding 40 kDa fragment of human plasma fibronectin purified from chymotryptic digest of human plasma fibronectin. This preparation contains the CS-1 heparin-binding region of fibronectin. Heparin-binding fragment promotes cytoskeleton organization in integrin signaling. This preparation shows one major band at 40 kDa in SDS-PAGE with no higher molecular weight species present. Smaller proteolytic fragments may be present.

      Human sourced raw material used in the production of this product tested negative for hepatitis B virus, hepatitis C virus (HCV), human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2), human T-cell lymphotropic virus (HTLV-1 and HTLV-2) and Treponema pallidum. Handle as if potentially infectious.
      References
      Product Information
      PresentationPurified by gelatin-Sepharose™ and heparin-Sepharose™ column chromatography. Lyophilized from sodium citrate buffered saline, 20mM, NaCl 150mM, pH 7.4. Reconstitute with distilled water.
      Quality LevelMQ100
      Applications
      Key Applications
      • Cell Culture
      Application NotesELISA

      Affinity chromatography

      Possibly for molecular mapping of fibronectin function

      Cell attachment and integrin signaling assays

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Concentration1 mg/mL
      SourceHuman
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
      Gene Symbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
      SIZE: 2386 amino acids; 262607 Da
      SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
      PTM: Sulfated.
      SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to one year from date of receipt. After reconstitution, maintain at -20°C for up to six months in undiluted aliquots. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      F1903 04053252271922

      Documentation

      Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified MSDS

      Title

      Safety Data Sheet (SDS) 

      Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified Certificates of Analysis

      TitleLot Number
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) PURIFIED PROTEIN - 2382066 2382066
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) - 3190963 3190963
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) - 3227949 3227949
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) - 3425714 3425714
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) - 3506670 3506670
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) - 3934925 3934925
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) - 4167100 4167100
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) PURIFIED PROTEIN 3045785
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) PURIFIED PROTEIN - 3752737 3752737
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) PURIFIED PROTEIN -2488809 2488809

      References

      Reference overviewPub Med ID
      Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
      Pierschbacher, M D, et al.
      Cell, 26: 259-67 (1981)  1981

      Show Abstract
      6174240 6174240
      Alignment of biologically active domains in the fibronectin molecule
      Ruoslahti, E. et al.
      J. Biol. Chem., 256:7277-7281 (1981)  1981

      6788765 6788765

      Data Sheet

      Title
      HUMAN FIBRONECTIN alpha-CHYMOTRYPTIC FRAGMENT 40kDa (heparin-binding) PURIFIED PROTEIN

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