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Merck

EPA102

EPA II Peroxidase Protection Buffer

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About This Item

eCl@ss:
42029053
UNSPSC Code:
41116010

Key Documents

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form

liquid

packaging

pkg of 500 mL

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

Quality Level

300

Application

500 mL EPA II Peroxidase Protection Buffer for ELISA & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

EPA II Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods.

Physical form

EPA II Buffer contains 0.5% (w/v) bovine serum albumin, antimicrobial preservatives and stabilizing compounds in phosphate buffered saline, pH 7.2.

Preparation Note

Store at 2° to 8°C, for up to 6 months.

Chemicon HRP conjugated antibodies may be diluted up to 1:16,000 in EPA II Buffer. Diluted conjugates are stable for at least 3 months when stored at 2° to 8°C. The buffer is suitable for direct use in E

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Luminata ELISA HRP Substrates

The Luminata ELISA HRP chemiluminescent substrates provide three major advantages: 1. High sensitivity for detecting as little as 0.1 pg of protein. 2. Broad dynamic detection range (up to 2,500-fold), enabling a wide range of quantitation. 3. Flexibility to adjust signal strength by one of two methods: a. Exposing the well to the luminometer for a longer time. b. Applying a more sensitive substrate to obtain signals in wells with otherwise undetectable signals.

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