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14-475 Cdk2/Cyclin E Protein, active, 10 µg

Active complex of recombinant full length human Cdk2 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag. For use in Kinase Assays.

Cdk2/Cyclin E Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-475
10 µg  Also available in 250μg size as catalog # 14-475M - Call for pricing and availability.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-475
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCdk2/Cyclin E Protein, active, 10 µg
      OverviewComplex of recombinant full length human cdk2 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive complex of recombinant full length human Cdk2 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceBoth are expressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
      Gene Symbol
      • CDK2
      • p33(CDK2)
      Protein TargetCDK2/cyclinE
      Purification MethodNi-NTA agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
      SIZE: 298 amino acids; 33930 Da
      SUBUNIT: Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1.
      SIMILARITY: SwissProt: P24941 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
      Molecular Weight34kDa (cdk2), 74kDa (cyclin
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of histone H1 by cdk2/cyclin E
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1year at -70C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size as catalog # 14-475M - Call for pricing and availability.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-475 04053252743689

      Documentation

      Cdk2/Cyclin E Protein, active, 10 µg MSDS

      Title

      Safety Data Sheet (SDS) 

      Cdk2/Cyclin E Protein, active, 10 µg Certificates of Analysis

      TitleLot Number
      Cdk2/cyclinE, active - 1851584-E 1851584-E
      Cdk2/cyclinE, active - 1851584-F 1851584-F
      Cdk2/cyclinE, active - 1901980-A 1901980-A
      Cdk2/cyclinE, active - 2166901-A 2166901-A
      Cdk2/cyclinE, active - D9AN017U-I D9AN017U-I

      FAQ

      QuestionAnswer
      Some of my enzyme preparations require Brij-35, what is its purpose?The role of Brij-35 is to function as a mild detergent, which prevents aggregation in active kinases.

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-475M Cdk2/Cyclin E Protein, active, 250 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases