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QIA70 Caspase-3 Activity Assay Kit, Fluorometric

Caspase-3 Activity Assay Kit, Fluorometric MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
QIA70
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityDetection Methods
      A Broad Range Of SpeciesFluorometric
      Description
      Overview

      This product has been discontinued.



      Suitable for the in vitro detection of caspase-3 activity in cell lysates. The assay is simple, rapid, and includes all controls. Suitable for use in frozen samples. Assay is validated against the Nucleosome ELISA Kit (Cat. No. QIA25) and the Cell Death Detection NMP ELISA Kit (Cat. No. QIA20). A high-throughput drug-screening version is also available (Cat. No. HTS02).
      Catalogue NumberQIA70
      Brand Family Calbiochem®
      Materials Required but Not Delivered• 1 M dithiothreitol (DTT, Cleland regent) solution (1.54 g to 10 ml H2O) made fresh (can be stored at -20°C for one month).
      • 2-20 µl, 20-200 µl and 200-1000 µl precision pipettes with disposable tips.
      • Fluorescent plate reader capable of measuring excitation ~400 nm, and emission at ~505 nm.
      • A centrifuge, for spinning tubes or 96-well plate and to pulse-spin the Recombinant Caspase-3 vials.
      • Microcentrifuge tubes.
      • Protease inhibitor cocktail set III (Cat. No. 539134)

      Note: It is recommended to add protease inhibitor cocktail at a 1:500 dilution to Sample Buffer and Assay Buffer just before use. These protease inhibitors have been shown to exhibit no effect on recombinant caspase-3 activity.
      References
      ReferencesX. Sun, et al. 1999. J. Biol. Chem. 274, 5053.
      N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312.
      S. J. Martin and D. 1995 R. Green, Cell 82, 349.
      Product Information
      Detection methodFluorometric
      Form96 Tests
      Format96-well plate
      Kit contains96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol.
      Quality LevelMQ100
      Applications
      Biological Information
      Assay time2.5-3 h
      Sample TypeCell lysates
      Species Reactivity
      • A Broad Range Of Species
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36-23

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Do not breathe fumes.
      Product Usage Statements
      Intended useThe Calbiochem Caspase-3 Activity Assay is a non-isotopic enzyme assay for the in vitro detection of Caspase-3 activity in cell lysates. The assay can be adapted for 96-well plates for sensitive drug screening methods.
      Storage and Shipping Information
      Ship Code Multiple Storage Conditions
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Storage ConditionsUpon Receipt of the kit: Store Recombinant Caspase-3 (2 vials) at -80°C to maintain stability. Store all other kit components at -20°C. Once thawed, Caspase-3 Sample Buffer and Assay Buffer may be stored at 4°C. Do not expose reagents to excessive light.
      Protect from Moisture Protect from moisture
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit contains96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol.
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      QIA70 0

      Documentation

      Caspase-3 Activity Assay Kit, Fluorometric MSDS

      Title

      Safety Data Sheet (SDS) 

      Caspase-3 Activity Assay Kit, Fluorometric Certificates of Analysis

      TitleLot Number
      QIA70

      References

      Reference overview
      X. Sun, et al. 1999. J. Biol. Chem. 274, 5053.
      N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312.
      S. J. Martin and D. 1995 R. Green, Cell 82, 349.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure

      Citations

      Title
    • So-Young Park and Adriana Ferreira. (2005) The generation of a 17 kDa neurotoxic fragment: an alternative mechanism by which Tau mediates β-amyloid-induced neurodegeneration. Journal of Neuroscience 25, 5365-5375.
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