BioTracker^™ Green pH-Independent Autophagy Live Cell Probe

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Fluorescence microscopyFlow cytometry

Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, dysfunctional proteins aggregate and are enclosed by a double membrane to form an autophagosome.

SCT276 is a small fluorescent molecule that detects autophagosomes and autolysosomes by a likely mechanism of incorporation into the autophagosome double membrane during its formation, whereupon it emits fluorescence under hydrophobic conditions. This probe has been noted to frequently colocalize with LC3. SPECTRAL CHARACTERISTICS: Excitation λ: 450 nmEmission λ: 535 nm

SCT276 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits green fluorescence under hydrophobic conditions. SCT276 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.Add 50 uL DMSO to tube of SCT276 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.

Store at -20 °C, protected from light.

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany