Millipore Sigma Vibrant Logo

69450 BL21(DE3) Competent Cells - Novagen

BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.

BL21(DE3) Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
69450
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
69450-3
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Glass bottle 0.4 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      69450-4
      Retrieving availability...
      —
      Limited Availability Limited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Glass bottle 1 ml
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          Description
          OverviewA common method for transformation of DNA plasmids into E. coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen prepared competent cells. Novagen competent cells represent the widest selection available for protein expression. Every Novagen competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.
          T7 expression strains are lysogens of bacteriophage DE3, as indicated by the (DE3). These hosts carry a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in appropriate T7 expression vectors, using IPTG as an inducer.
          BL21 has been the gold standard for protein expression since it was first introduced in 1990. Deficient in lon and ompT proteases, BL21 and its derivatives are ideal for many applications.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number69450
          Brand Family Novagen®
          References
          Product Information
          Components
          Guaranteed efficiency> 2 x 10⁷ cfu/μg
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          69450-3 04055977274332
          69450-4 04055977274349

          Documentation

          BL21(DE3) Competent Cells - Novagen MSDS

          Title

          Safety Data Sheet (SDS) 

          BL21(DE3) Competent Cells - Novagen Certificates of Analysis

          TitleLot Number
          69450

          Brochure

          Title
          Competent Cell Brochure

          Citations

          Title
        • Chen, X., et al. 2008. Procedings of the National Academy of Science 105, 4453.
        • Clock, S.A., et al. 2008. Journal of Bacteriology 190, 980.
        • Herskowitz, J.H., et al. 2008. Journal of Virology 82, 3295.
        • Shelburne, III, S.A., et al. 2008. Procedings of the National Academy of Science 105, 1698.
        • Li, G-q., et al. 2008. Applied and Enviornmental Microbiology 74, 971.
        • Li, F. et al. 2008. Plant Physiology 146, 1333.
        • Ian T. Major and C. Peter Constabel. 2008. Plant Physiology 146, 888.
        • Masip, L., et al. 2008. Journal of Biological Chemistry 283, 840.
        • Joanne C. McGrail and Raymond T. O'Keefe. 2008. Nucleic Acids Research 36, 814.
        • Robertson, P.D., et al. 2008. Journal of Biological Chemistry 283, 3338.
        • Rossi, R., et al. 2008. Journal of Biological Chemistry 283, 3559.
        • Zhang, L., et al. 2008. Blood 111, 1193.
        • Ang, E.L., et al. 2007. Federation of American Societies for Experimental Biology Journal 274, 928.
        • Cai, M., et al. 2007. Journal of Biological Chemistry 282, 14525.
        • Edner, C., et al. 2007. Plant Physiology 145, 17.
        • Fukuda, C., et al. 2007. Applied and Enviornmental Microbiology 73, 5447-.
        • Guan, C., et al. 2007. Applied and Enviornmental Microbiology 73, 3669-.
        • Alexander F. Jeans, A.F., et al. 2007. Procedings of the National Academy of Science 104, 2431.
        • Lu, H.A.J., et al. 2007. Journal of Biological Chemistry 282, 28721.
        • Nishiwaki, H., et al. 2007. Applied and Enviornmental Microbiology 73, 3404-.
        • MichelOtte, M.M., et al. 2007. Journal of Bacteriology 189, 7310.
        • Park, M.-O., et al. 2007. Journal of Bacteriology 189, 7281.
        • Qi, M., et al. 2007. Applied and Enviornmental Microbiology 73, 6098.
        • Shchedrina, V.A., et al. 2007. Procedings of the National Academy of Science 104, 13919.
        • Strube, K., et al. 2007. Journal of Biological Chemistry 282, 20292.
        • Satoshi Tasumi and Gerardo R. Vasta. 2007. Journal of Immunology 179, 3086.
        • Yang, F., et al. 2007. Journal of Biological Chemistry 282, 3856.
        • Yang, M., et al. 2007. Cell , 744.
        • Zhao, K.-H., et al. 2007. Procedings of the National Academy of Science 104, 14300.
        • Gakh, O., et al. 2006. Human Molecular Genetics 15, 467.
        • Heraud, J.-M., et al. 2006. Journal of immunology 177, 2552.
        • Janjic, B., et al. 2006. Journal of Immunology 177, 2717.
        • Alex Kudrin, A., et al. 2006. Journal of Biological Chemistry 281, 29641.
        • Park, S.-A., et al. 2006. Procedings of the National Academy of Science 103, 11886.
        • Rachelle M. Smith and Stanly B. Williams. 2006. Proceedings of the National Academy of Sciences (USA) 103, 8564.
        • Sosnovtsev, S.V., et al. 2006. Journal of Virology 80, 7816.
        • Stewart, J.J., et al. 2006. Journal of Biological Chemistry 281, 22665.
        • Alphey, M.S., et al. 2005. Journal of Biological Chemistry 280, 3068.
        • Christophe Anjard and William F. Loomis. 2005. Procedings of the National Academy of Science 102, 7607.
        • Bachler, C., et al. 2005. European Molecular Biology Organization Journal 24, 283.
        • Banki, M.,R., et al. 2005. Protein Science 14, 1387.
        • Bausch, S.L., et al. 2005. Journal of Biological Chemistry 280, 29956.
        • Birgander, P.L., et al. 2005. Journal of Biological Chemistry 280, 14997.
        • Bonner, E.R., et al. 2005. Journal of Biological Chemistry 280, 38803.
        • Leisl A. Brand and Erick Strauss. 2005. Journal of Biological Chemistry 280, 20185.
        • Brouns, S.J.J., et al. 2005. Journal of Biological Chemistry 280, 11422.
        • Bruck, I., et al. 2005. Journal of Biological Chemistry 280, 18152.
        • van Bueren, A.L., et al. 2005. Journal of Biological Chemistry 280, 530.
        • Carmelo, E., et al. 2005. Journal of Biological Chemistry 280, 28683.
        • Casarosa, P., et al. 2005. Journal of Biological Chemistry 280, 3275.
        • Pedro Castanheira, P., et al. 2005. Journal of Biological Chemistry 280, 13047.
        • Chen, M-H., et al. 2005. Journal of Biological Chemistry 280, 10599.
        • Veronika Csizmok, V., et al. 2005. Molecular and Cellular Proteomics 5, 265.
        • Davidson, D.J., et al. 2005. Cancer Research 65, 4663.
        • Eggler, A.L., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 10070.
        • Fierobe, H-P., et al. 2005. Journal of Biological Chemistry 280, 16325.
        • Flaig, R., et al. 2005. Journal of Biological Chemistry 280, 19250.
        • Francis, K., et al. 2005. Journal of Biological Chemistry 280, 5195.
        • Fukuda, A., et al. 2005. Plant and Cell Physiology 46, 1779.
        • Gómez-Merino, F.C., et al. 2005. Journal of Biological Chemistry 280, 34888.
        • Georgescu, S.P., et al. 2005. Molecular Endocrinology 19, 2491.
        • Peter Gin and Catherine F. Clarke. 2005. Journal of Biological Chemistry 280, 2676.
        • Guo, R-T., et al. 2005 Journal of Biological Chemistry 280, 20762.
        • Han, J-M., et al. 2005. Journal of Lipid Research 46, 589.
        • Hanaoka, S., et al. 2005. Protein Science 14, 119.
        • Herman, P.L., et al. 2005. Journal of Biological Chemistry 280, 24759.
        • Heurgue-Hamard, V., et al. 2005. Journal of Biological Che
          		•

          User Protocols

          Title
          TB009 Competent Cells
          TB053 Academic and Non-profit Laboratory Assurance Letter

          Related Products & Applications

          Related Products

          Catalogue Number Description  
          69449 BL21 Competent Cells - Novagen Show Pricing & Availability
          69451 BL21(DE3)pLysS Competent Cells - Novagen Show Pricing & Availability
          70232 BL21 Competent Cell Set - Novagen Show Pricing & Availability

          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression