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ABS1004 Anti-phospho-NKCC1 Antibody (Thr212/Thr217)

Anti-phospho-NKCC1 Thr212/Thr217 is an antibody against phospho-NKCC1 for use in Western Blotting, Immunofluorescence.

Anti-phospho-NKCC1 Antibody (Thr212/Thr217) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: SLC12A2, NKCC1 UniProt Number: P55011
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ABS1004
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IFRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1004
      DescriptionAnti-phospho-NKCC1 Antibody (Thr212/Thr217)
      Alternate Names
      • phospho-NKCC1 Thr212/Thr217
      • Solute carrier family 12 member 2
      • Basolateral Na-K-Cl symporter
      • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
      Background InformationThe Na-K-Cl cotransporter 1 (NKCC1) is a membrane protein coded by the SLC12A2 gene and functions in cellular sodium/potassium/chloride transport and homeostasis. It is widely distributed throughout the body and serves important functions, especially in liquid-secreting glands. Phosphorylation of Thr212/Thr217 in the intracellular N-terminal domain of NKCC1 is correlated with NKCC1 activation.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-NKCC1 Thr212/Thr217 is an antibody against phospho-NKCC1 for use in Western Blotting, Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GMB43 and GBM5 cell lysate (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31).
      Immunofluorescence Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GBM43 human tissiue (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31).
      Biological Information
      ImmunogenLinear peptide corresponding to human phospho-NKCC1 Thr212/Thr217.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificitySpecific for mature form of phosphorylated NKCC1.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC12A2
      • NKCC1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~250 kDa observed. Likely a dimer of mature form of NKCC1, NKCC1 is glycosylated differently in different cells, leading to uncharacterized band(s) in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in GMB43 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-NKCC1 Thr212/Thr217 in 10 µg of GMB43 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1004 04055977133332

      Documentation

      Anti-phospho-NKCC1 Antibody (Thr212/Thr217) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-NKCC1 Antibody (Thr212/Thr217) Certificates of Analysis

      TitleLot Number
      Anti-phospho-NKCC1 Thr212/Thr217 - 3224588 3224588
      Anti-phospho-NKCC1 Thr212/Thr217 - 3474199 3474199
      Anti-phospho-NKCC1 Thr212/Thr217 - 3726147 3726147
      Anti-phospho-NKCC1 Thr212/Thr217 - 3909656 3909656
      Anti-phospho-NKCC1 Thr212/Thr217 - 4045821 4045821
      Anti-phospho-NKCC1 Thr212/Thr217 - 4123894 4123894
      Anti-phospho-NKCC1 Thr212/Thr217 - 4149291 4149291
      Anti-phospho-NKCC1 Thr212/Thr217 - 4216369 4216369
      Anti-phospho-NKCC1 Thr212/Thr217 -2634209 2634209
      Anti-phospho-NKCC1 Thr212/Thr217 -2645985 2645985
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      References

      Reference overviewPub Med ID
      The Journal of endocrinology
      Tiptaft, EM; Jones, MT
      The Journal of endocrinology  64  9P-10P  1975

      		163881 163881

      Brochure

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      Technical Info

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      White Paper: Further considerations of antibody validation and usage.