Millipore Sigma Vibrant Logo

MABE325 Anti-p21WAF1 Antibody, clone EA10

View Products on Sigmaaldrich.com
MABE325
100 µg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE325
      DescriptionAnti-p21WAF1 Antibody, clone EA10
      Alternate Names
      • Cyclin-dependent kinase inhibitor 1
      • CDK-interacting protein 1
      • Melanoma differentiation-associated protein 6
      • MDA-6
      • p21
      Background InformationThe tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ~21 kDa product of the WAF1 gene is found in a complex involving cyclins, CDKs, and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is the prevention of CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. While the role of p21WAF1 in apoptosis is less clear, it is known that p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 can occur by both p53-dependent and p53-independent mechanisms, in response to certain observed conditions. p21WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Its overexpression was observed to inhibit cellular growth.
      Product Information
      FormatPurified
      Control
      • HT-29 cell lysate
      PresentationPurified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-p21WAF1 Antibody, clone EA10 is a Mouse Monoclonal Antibody for detection of p21WAF1 & has been validated in WB, FC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesWestern Blot Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p21WAF1 in normal human lower back tissue (el-Deiry, W. S., et al. (1995). Cancer Res. 55(13):2910-2919.).

      Flow Cytometry Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428).
      Biological Information
      ImmunogenRecombinant protein corresponding to human p21WAF1.
      CloneEA10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDKN1A
      • CAP20
      • CDKN1
      • CIP1
      • MDA6
      • PIC1
      • SDI1
      • WAF1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~21 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HT-29 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected p21WAF1 in 10 µg of HT-29 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABE325 04053252691560