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PC409 Anti-p14ARF (Ab-1) (118-132) Rabbit pAb

This Anti-p14ARF (Ab-1) (118-132) Rabbit pAb is validated for use in Immunoblotting for the detection of p14ARF (Ab-1) (118-132).

Anti-p14ARF (Ab-1) (118-132) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC409
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.




      Recognizes the ~14 kDa p14ARF protein in Saos-2 cells. Sold under license of U.S. Patent 6,482,929.
      Catalogue NumberPC409
      Brand Family Calbiochem®
      Application Data
      Detection of human p14ARF by immunoblotting. Sample: Whole cell lysate from SAOS cells. Primary antibody: Anti-p14ARF (Ab-1) (118-132) Rabbit pAb (1:500). Detection: chemiluminescence.
      References
      ReferencesSharpless, N.E., and DePinho, R. A., 1999. Curr. Opin. Genet. Devel. 9, 22.
      Bates, S., et al. 1998. Nature 395, 124.
      Gorgoulis, V.G., et al. 1998. Am. J. Pathol. 153, 1749.
      Pomerantz, J., et al. 1998. Cell 92, 713.
      Stott, F.J., et al. 1998. EMBO J. 17, 5001.
      Vonlanthen, S., et al. 1998. Oncogene 17, 2770.
      Zhang, Y., et al. 1998. Cell 92, 725.
      Product Information
      DeclarationSold under license of U.S. Patent 6,482,929.
      FormLiquid
      FormulationUndiluted serum.
      Positive controlSaos-2 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:500)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide [(K)AGRARCLGPSARGPG] corresponding to amino acids 118-132 of human p14ARF (p16β)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC409 0

      Documentation

      Anti-p14ARF (Ab-1) (118-132) Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p14ARF (Ab-1) (118-132) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC409

      References

      Reference overview
      Sharpless, N.E., and DePinho, R. A., 1999. Curr. Opin. Genet. Devel. 9, 22.
      Bates, S., et al. 1998. Nature 395, 124.
      Gorgoulis, V.G., et al. 1998. Am. J. Pathol. 153, 1749.
      Pomerantz, J., et al. 1998. Cell 92, 713.
      Stott, F.J., et al. 1998. EMBO J. 17, 5001.
      Vonlanthen, S., et al. 1998. Oncogene 17, 2770.
      Zhang, Y., et al. 1998. Cell 92, 725.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-October-2007 RFH
      ApplicationImmunoblotting (1:500)
      Application Data
      Detection of human p14ARF by immunoblotting. Sample: Whole cell lysate from SAOS cells. Primary antibody: Anti-p14ARF (Ab-1) (118-132) Rabbit pAb (1:500). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~14 kDa p14ARF protein.
      BackgroundThe human p14ARF protein (murine homologue is p19ARF) results from the translation of an alternative reading frame of the CDKN2A (p16INK4) gene. The α transcript gives rise to the p16 gene product while the β transcript gives rise to the p14ARF gene product. Although transcriptionally derived from shared exons, there is little amino acid sequence homology between the two proteins. The function of the p14ARF protein is to activate a p53 response and thereby induce cell cycle arrest. p53 in turn negatively regulates p14ARF expression. The mechanism of action for p14ARF is a direct binding of the COOH terminal domain of MDM2 which in turn forms a ternary complex with p53. Interaction with MDM2 results in the blocking of the MDM2 induced degradation of p53. p14ARF transcription is also modulated by the activity of E2F1 expression. Loss of p14ARF correlates with loss of p16 expression and alteration in expression of both proteins is frequently seen in non-small cell lung carcinoma.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide [(K)AGRARCLGPSARGPG] corresponding to amino acids 118-132 of human p14ARF (p16β)
      IsotypeIgG
      Specieshuman, not mouse, not rat
      Positive controlSaos-2 cells
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSharpless, N.E., and DePinho, R. A., 1999. Curr. Opin. Genet. Devel. 9, 22.
      Bates, S., et al. 1998. Nature 395, 124.
      Gorgoulis, V.G., et al. 1998. Am. J. Pathol. 153, 1749.
      Pomerantz, J., et al. 1998. Cell 92, 713.
      Stott, F.J., et al. 1998. EMBO J. 17, 5001.
      Vonlanthen, S., et al. 1998. Oncogene 17, 2770.
      Zhang, Y., et al. 1998. Cell 92, 725.