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MABE1076 Anti-mono- ADP-ribose binding reagent

Anti-mono-ADP-ribose binding reagent, Cat. No. MABE1076, is a reagent that targets mono (ADP-ribose) modified proteins and has been tested in Dot Blot and Western Blotting.

Anti-mono- ADP-ribose binding reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function UniProt Number: N/A
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MABE1076
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HDB, WBE. coliPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1076
      DescriptionAnti-mono- ADP-ribose binding reagent
      Background InformationAnti-mono-ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
      References
      Product Information
      FormatPurified
      PresentationPurified from E. coli and supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM beta-Mercaptoethanol, with 10% glycerol without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-mono-ADP-ribose binding reagent, Cat. No. MABE1076, is a reagent that targets mono (ADP-ribose) modified proteins and has been tested in Dot Blot and Western Blotting.
      Key Applications
      • Dot Blot
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.4 µg/mL of a representative lot detected mono-ADP-ribosylated proteins.

      Dot Blot Analysis: A representative lot detected mono-,-ADP-ribose modified proteins.

      Dot Blot Analysis: A representative lot detected mono-ADP ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).

      Western Blotting Analysis: A representative lot detected mono-ADP-ribose modified protein by Wester blotting (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
      Biological Information
      ConcentrationPlease refer to lot specific datasheet.
      HostE. coli
      SpecificityThis reagent binds to mono-ADP ribosylated proteins.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human.
      Antibody TypeMonoclonal Antibody
      Modifications
      • ADP-ribosylation
      Purification MethodImmobilized Metal Affinity Chromatography (IMAC)
      UniProt Number
      Molecular WeightVariable depending on the target proteins and the extent of ADP-ribosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Gel Electrophoresis.


      Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1076 04054839330711

      Documentation

      Anti-mono- ADP-ribose binding reagent MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-mono- ADP-ribose binding reagent Certificates of Analysis

      TitleLot Number
      Anti-mono ADP Ribose Binding Reagent - 3044949 3044949
      Anti-mono ADP Ribose Binding Reagent - 3062746 3062746
      Anti-mono ADP Ribose Binding Reagent - 3167553 3167553
      Anti-mono ADP Ribose Binding Reagent - 3389888 3389888
      Anti-mono ADP Ribose Binding Reagent - 3689905 3689905
      Anti-mono ADP Ribose Binding Reagent - 3870878 3870878
      Anti-mono ADP Ribose Binding Reagent - 3929307 3929307
      Anti-mono ADP Ribose Binding Reagent - 4033486 4033486
      Anti-mono ADP Ribose Binding Reagent - 4080437 4080437
      Anti-mono ADP Ribose Binding Reagent - 4095331 4095331
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