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MABN1777-100UG Anti-VAChT Antibody, clone N425/45

Anti-VAChT, clone N425/45, Cat. No. MABN1777, is a mouse monoclonal antibody that detects Vesicular acetylcholine transporter (VAChT) and is tested for use in Immunohistochemistry (Paraffin).

MABN1777-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN1777-100UG
      DescriptionAnti-VAChT Antibody, clone N425/45
      Alternate Names
      • Vesicular acetylcholine transporter
      • rVAT
      • Solute carrier family 18 member 3
      Background InformationVesicular acetylcholine transporter (UniProt: Q62666; also known as VAChT, rVAT, Solute carrier family 18 member 3) is encoded by the Slc18a3 (also known as Vacht, Vat) gene (Gene ID: 60422) in rat. Acetylcholine (ACh) is synthesized in nerve terminals from choline and acetyl coenzyme A by the cytoplasmic choline acetyltransferase. ACh is then transported into synaptic vesicles where it is stored until release. This transport from cytoplasm into synaptic vesicles is achieved via vesicular acetylcholine transporter (VAChT) that acts as an antiporter to couple ACh entry into synaptic vesicles with proton efflux. VAChT is a mutli-pass membrane protein with seven cytoplasmic domains, twelve transmembrane domains, and six lumenal domains. High expression of VAChT is observed in all major cholinergic neurons, including postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord, and brainstem motoneurons. It is weakly expressed in the cortex and hippocampus. Elevated levels of VAChT are reported to affect its own intraneuronal trafficking and can remodel striatal cholinergic interneuron morphology and reduce the number and increase the size of cholinergic varicosities. (Ref.: Arvidsson, U., et al. (1997). J. Comp. Neurol. 378(4); 454-467; Janickova, H., et al. (2017). J. Nurochem. 142(6); 857-875).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-VAChT, clone N425/45, Cat. No. MABN1777, is a mouse monoclonal antibody that detects Vesicular acetylcholine transporter (VAChT) and is tested for use in Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenRecombinant fragment corresponding to the first lumenal domain of rat Vesicular acetylcholine transporter.
      EpitopeN-terminal
      CloneN425/45
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone N425/45 is a mouse monoclonal antibody that detects Vesicular acetylcholine transporter. It targets an epitope within the first lumenal region.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slc18a3
      • Vacht
      • Vat
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight56.54 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in rat brain tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected VAChT in rat brain tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1777-100UG 04061841879695