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ST1200 Anti-Ubiquitin Mouse mAb (FK2)

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.

Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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ST1200
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

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ST1200-100UG
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      Description
      OverviewRecognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
      Catalogue NumberST1200
      Brand Family Calbiochem®
      Application Data
      Detection of ubiquitin by immunoblotting. Samples: K48-linked ubiquitin chains (lanes A and D), K29-linked ubiquitin chains (lanes B and E), and K63-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
      References
      ReferencesMatsumoto, M., et al. 2005. Proteomics 5, 4545.
      Boname, J.N., et al. 2001. Immunity 15, 627.
      Campbell, V., et al. 2001. Neuron 32, 1013.
      Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesELISA Takada, K., et al. 1995. Eur. J. Biochem. 233, 42. Takada, K., et al. 1997. Clin. chem. 43, 1188. Immunoprecipitation Takada, K., et al. 2001. Biochem. J. 356, 199. Boname, J.N. and Stevenson, P.G. 2001. Immunity 15, 627. Matsumoto, M., et al. 2005. Proteomics 5, 4145. Immunofluorescence Campbell, D.S. and Holt, C.E. 2001. Neuron 32, 1013.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesELISA (see application references)
      Immunoblotting (1:1000)
      Immunoprecipitation (see application references and comments)
      Immunofluorescence (see application references)
      Application CommentsDoes not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenunpurified polyubiquitinylated lysozyme
      Immunogenhuman
      CloneFK2
      HostMouse
      IsotypeIgG₁
      SourceRaji cells
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1200-100UG 04055977224078

      Documentation

      Anti-Ubiquitin Mouse mAb (FK2) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Ubiquitin Mouse mAb (FK2) Certificates of Analysis

      TitleLot Number
      ST1200

      References

      Reference overview
      Matsumoto, M., et al. 2005. Proteomics 5, 4545.
      Boname, J.N., et al. 2001. Immunity 15, 627.
      Campbell, V., et al. 2001. Neuron 32, 1013.
      Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-March-2009 JSW
      ApplicationELISA (see application references)
      Immunoblotting (1:1000)
      Immunoprecipitation (see application references and comments)
      Immunofluorescence (see application references)
      Application Data
      Detection of ubiquitin by immunoblotting. Samples: K48-linked ubiquitin chains (lanes A and D), K29-linked ubiquitin chains (lanes B and E), and K63-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.
      HostMouse
      Immunogen specieshuman
      Immunogenunpurified polyubiquitinylated lysozyme
      CloneFK2
      IsotypeIgG₁
      Speciesa broad range of species
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      SourceRaji cells
      Preservative≤0.1% sodium azide
      CommentsDoes not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
      Toxicity Standard Handling
      ReferencesMatsumoto, M., et al. 2005. Proteomics 5, 4545.
      Boname, J.N., et al. 2001. Immunity 15, 627.
      Campbell, V., et al. 2001. Neuron 32, 1013.
      Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
      Application referencesELISA Takada, K., et al. 1995. Eur. J. Biochem. 233, 42. Takada, K., et al. 1997. Clin. chem. 43, 1188. Immunoprecipitation Takada, K., et al. 2001. Biochem. J. 356, 199. Boname, J.N. and Stevenson, P.G. 2001. Immunity 15, 627. Matsumoto, M., et al. 2005. Proteomics 5, 4145. Immunofluorescence Campbell, D.S. and Holt, C.E. 2001. Neuron 32, 1013.