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05-829-AF555 Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate

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05-829-AF555
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Gp, Av, B, Porcine, Gr, RICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue Number05-829-AF555
      DescriptionAnti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate
      Alternate Names
      • Tubulin alpha-1 chain
      • Alpha-tubulin
      • Alpha-1 tubulin
      Background InformationTubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tubes. Various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of α-Tubulin.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
      Biological Information
      ImmunogenPurified chicken brain α-tubulin.
      EpitopeC-terminal 25 amino acids.
      CloneDM1A
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Human
      • Guinea Pig
      • Avian
      • Bovine
      • Porcine
      • Gerbil
      • Rat
      Species Reactivity NoteMouse, Human, Guinea Pig, Avian, Bovine, Porcine, Gerbil, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBA1C
      • TUBA1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~50 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-tubulin in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-829-AF555 04055977355550

      Documentation

      Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 555 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 3120123 3120123
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 3231926 3231926
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 3425404 3425404
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 3738006 3738006
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 4078570 4078570
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate - 4139150 4139150
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 555 Conjugate -Q2696985 Q2696985

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies