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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9F8, monoclonal
Application:
ELISA, ICC, IHC, WB
Citations:
2
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
9F8, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TNC(3371)
Immunogen
Epitope: type III repeats 1-3
Purified human tenascin
Application
Immunohistochemistry at 2 μg/mL on tissues fixed with cold acetone
Immunocytochemistry at 2 μg/mL on U251 cells
Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions
ELISA: 2 x 10-10 M endpoint dilution
Affinity constant Kd= 6.37 x 10-9
Final working dilutions must be determined by end user.
Immunocytochemistry at 2 μg/mL on U251 cells
Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions
ELISA: 2 x 10-10 M endpoint dilution
Affinity constant Kd= 6.37 x 10-9
Final working dilutions must be determined by end user.
This Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8 is validated for use in ELISA, WB, IC, IH for the detection of Tenascin.
Biochem/physiol Actions
Binds to a recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 1-3.
Physical form
Format: Purified
Liquid in 0.02 M phosphate, 0.25 M Sodium Chloride pH 7.6, with 0.1% Sodium Azide.
Preparation Note
Store at 2 and 8°C for up to 6 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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wgk
WGK 2
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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