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MABN2394 Anti-Syntaxin-3B Antibody, clone 12E5

Anti-Syntaxin-3B clone 12E5, Cat. No. MABN2394, is a highly specific mouse monoclonal antibody that targets Syntaxin 3B and has been tested in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-Syntaxin-3B Antibody, clone 12E5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Stx3 UniProt Number: Q64704
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MABN2394
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, BICC, WB, IHC, IP, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2394
      DescriptionAnti-Syntaxin-3B Antibody, clone 12E5
      Alternate Names
      • STX3
      Background InformationSyntaxin-3 (UniProt: Q64704; also known as STX3) is encoded by the Stx3 gene (Gene ID: 20908) in murine species. Syntaxin-3B is a single-pass type IV membrane protein that is specific for ribbon synapses of the retina. Stx3 gene codes for four different splice forms, Syntaxin 3A, 3B, 3C and 3D. Syntaxin 3A and 3B have an identical N-terminal domain but differ in the C-terminal half of the SNARE domain and the C-terminal transmembrane domain. These two domains are thought to be directly involved in synaptic vesicle fusion. Syntaxin 3 protein is found in all ribbon synapses of the retina. This type of synapse in the retina is formed by rod and cone photoreceptors, as well as by bipolar neurons. Syntaxin 3B has been shown to be important for synaptic vesicle exocytosis in retinal bipolar cells indicating a specific role for Syntaxin 3B in ribbon synapses. Syntaxin 3B can be phosphorylation at Thr14 by CAM Kinase II is shown to increase its binding to SNAP-25. (Ref.: Curtis, LB et al. (2008). J. Comp. Neurol. 510(5): 550-559; Liu, X et al. (2014). Mol. Cell. Neurosci. 60(1): 53-62).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Syntaxin-3B clone 12E5, Cat. No. MABN2394, is a highly specific mouse monoclonal antibody that targets Syntaxin 3B and has been tested in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Syntaxin-3B in human retina and mouse eye tissues.

      Western Blotting Analysis: A representative lot detected Syntaxin-3B in Western Blotting applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

      Immunoprecipitation Analysis: A representative lot detected Syntaxin-3B in Immunoprecipitation applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

      Immunohistochemistry Analysis: A representative lot detected Syntaxin-3B in Immunohistochemistry applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).

      Immunocytochemistry Analysis: A representative lot detected Syntaxin-3B in Immunocytochemistry applications (Zulliger, R., et. al. (2015). PLoS One. 10(9):e0138508).
      Biological Information
      ImmunogenGST-tagged recombinant murine Syntaxin 3, isoform B that lacks the transmembrane domain.
      Clone12E5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 12E5 is a highly specific antibody against the B isoform of Syntaxin 3, which is abundantly expressed in photoreceptors of the retina.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Bovine
      Species Reactivity NoteBovine, Human, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Stx3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~33 kDa observed; 32.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human retinal tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Syntaxin-3B in 10 µg of human retinal tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2394 04054839197741