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MABS1911-25UL Anti-Serpin B9 Antibody, clone 7D8

Anti-Serpin B9, clone 7D8, Cat. No. MABS1911, is a mouse monoclonal antibody that detects Serpin B9 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.

Research Category: Inflammation & Immunology Gene Symbol: SERPINB9, PI9 UniProt Number: P50453
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MABS1911-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, FC, ICC, IH(P), IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1911-25UL
      DescriptionAnti-Serpin B9 Antibody, clone 7D8
      Alternate Names
      • Cytoplasmic antiproteinase 3
      • CAP-3
      • CAP3
      • Peptidase inhibitor 9
      • PI-9
      Background InformationSerpin B9 (UniProt: P50453; also known as Cytoplasmic antiproteinase 3, CAP-3, CAP3, Peptidase inhibitor 9, PI-9) is encoded by the SERPINB9 (also known as PI9) gene (Gene ID: 5272) in human. Serpins serve as intracellular or extracellular regulators of a wide range of physiological processes such as complement activation, blood coagulation and apoptosis. Within the vertebrate immune system, they control proteases of the classical and innate complement systems, and are also potent inhibitors of many leukocyte granule proteases. Serpin B9 has a broad tissue distribution, being present at high and relatively stable levels natural killer cells and CD8+ T cells, antigen-presenting cells, in many endothelial and mesothelial cells and at sites of immune privilege such as testis and placenta. Serpin B9 serves as a regulator granzyme B activity by acting as an inhibitor of its activity. Overexpression of Serpin B9 may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. Certain tumors may upregulate Serpin B9 levels as a mechanism of immune evasion. (Ref.: Kaiserman, D., and Bird, PI (2010). Cell Death Differen. 17(4); 586-595).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Serpin B9, clone 7D8, Cat. No. MABS1911, is a mouse monoclonal antibody that detects Serpin B9 and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Western Blotting
      Application NotesELISA Analysis: A representative lot detected Serpin B9 in ELISA applications (Hirst, C.E., et. al. (2001). Mol Hum Peprod. 7(12):1133-42).

      Flow Cytometry Analysis: A representative lot detected Serpin B9 in Flow Cytometry applications (Hirst, C.E., et. al. (2003). J Immunol. 170(2):805-15).

      Immunocytochemistry Analysis: A representative lot detected Serpin B9 in Immunocytochemistry applications (Hirst, C.E., et. al. (2003). J Immunol. 170(2):805-15).

      Immunofluorescence Analysis: A representative lot detected Serpin B9 in Immunofluorescence applications (Hirst, C.E., et. al. (2003). J Immunol. 170(2):805-15).

      Immunohistochemistry Analysis: A representative lot detected Serpin B9 in Immunohistochemistry applications (Hirst, C.E., et. al. (2001). Mol Hum Peprod. 7(12):1133-42).

      Western Blotting Analysis: A 1:100 dilution from a representative lot detected Serpin B9 in YT and Jurkat cell lysates (Courtesy of Prof. Phillip Bird, Ph.D., Monash University, Victoria Australia).

      Western Blotting Analysis: A representative lot detected Serpin B9 in Western Blotting applications (Hirst, C.E., et. al. (2001). Mol Hum Peprod. 7(12):1133-42; Hirst, C.E., et. al. (2003). J Immunol. 170(2):805-15; Mangan, M.S., et. al. (2015). J Biol Chem. 291(7):3626-38).

      Immunocytochemistry Analysis: A representative lot detected Serpin B9 in COS-1 cells (Courtesy of Prof. Phillip Bird, Ph.D., Monash University, Victoria Australia).
      Biological Information
      ImmunogenHis-tagged full length human recombinant Serpin B9.
      Clone7D8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7D8 detects Serpin B9 in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SERPINB9
      • PI9
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight42.40 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human spleen tissue sections.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Serpin B9 in human spleen tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1911-25UL 04054839441172

      Documentation