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ABN1662 Anti-STRA6

Anti-STRA6, Cat. No. ABN1662, is a highly specific rabbit polyclonal antibody that targets Stimulated by retinoic acid gene 6 protein (STRA6) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-STRA6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Stra6 UniProt Number: O70491
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ABN1662
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, IH(P), IP, WBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1662
      DescriptionAnti-STRA6
      Alternate Names
      • Stimulated by retinoic acid gene 6 protein
      • Retinoic acid-responsive protein
      Background InformationStimulated by retinoic acid gene 6 protein (UniProt: O70491; also known as Retinoic acid-responsive protein, STRA6) is encoded by the Stra6 gene (Gene ID: 20897) in murine species. STRA6 is a multi-pass membrane protein that acts as a high-affinity cell-surface receptor for the complex retinol-retinol binding protein (RBP/RBP4). It has one intramembrane and nine transmembrane helices in a dimeric assembly. It acts by removing retinol from RBP/RBP4 and transports it across the plasma membrane, where it can be metabolized. This mechanism does not depend on endocytosis. Binds to RBP/RBP4 with high affinity. Increases cellular retinol uptake from the retinol-RBP complex. STRA6 is widely expressed in the embryo and it in adults it is expressed choroid plexus and the brain microvascular cells that form the blood-organ barrier. It is also expressed in the retinal pigment epithelium, seminiferous epithelium in testis, the yolk sac, and in chorioallantoic placenta. Its expression in Sertoli cells depends on the stage of the spermatogenic cycle. STRA6 can be induced by retinoic acid and it is shown to be synergistically up-regulated by Wnt1 and retinoid in mammary epithelial cells. Mutations in Stra6 gene have been linked to the Matthew-Wood syndrome that is characterized by pulmonary agenesis/hypoplasia; microphthalmia/anophthalmia; and congenital cardiac, digestive, and urogenital malformations. (Ref.: Sadowski, S et al. (2017). Birth Defects Res. 109(4); 251-253; Chen, Y et al. (2016). Science 353 (6302), aad8266).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-STRA6, Cat. No. ABN1662, is a highly specific rabbit polyclonal antibody that targets Stimulated by retinoic acid gene 6 protein (STRA6) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot detected STRA6 in 293T cells transfected with an empty vector or pCMVTag2b-Stra6 (Courtesy of Dr. Noa Noy).

      Western Blotting Analysis: A representative lot detected STRA6 in Western Blotting applications (Berry, D.C., et. al. (2012). Mol Cell Biol. 32(19):3851-9).

      Western Blotting Analysis: A representative lot detected STRA6 in 293T cells transfected with an empty vector or pCMVTag2b-Stra6 (Courtesy of Dr. Noa Noy).

      Flow Cytometry Analysis: A 1:100 dilution from a representative lot detected STRA6 in HT-29 cells.

      Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected STRA6 in mouse testis and human liver cancer tissues.
      Biological Information
      ImmunogenHis-tggged recombinant fragment corresponding to 109 amino acids from the C-terminal region of murine Stimulated by retinoic acid gene 6 (STRA6) protein.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects murine Stimulated by retinoic acid gene 6 protein (STRA6). it targets an epitope within 109 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Stra6
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~90 kDa observed; 73.77 kda calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3/L1Adipocytes.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected STRA6 in NIH3T3/L1 Adipocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1662 04054839396748