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MABF3074-100UG Anti-SRF Antibody, clone 2C5

Anti-SRF, clone 2C5, Cat. No. MABF3074, is a rat monoclonal antibody that detects serum response factor (SRF) and is used in Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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MABF3074-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3074-100UG
      DescriptionAnti-SRF Antibody, clone 2C5
      Alternate Names
      • Serum response factor
      Background InformationSerum response factor (UniProt: Q9JM73; also known as SRF) is encoded by the Srf gene (Gene ID: 20807) in murine species. SRF is a transcription factor that binds to a serum response element (SRE) associated with a variety of genes, including immediate early genes such as c-Fos, FosB, JunB, Egr-1 and -2; neuronal genes such as nurr1 and nur77. It binds to DNA as a dimer and its DNA binding region is localized in amino acids 129-218. By regulating the expression of several genes, it controls cell growth and differentiation, neuronal transmission, and muscle development. Together with Myocardin Related Transcription Factor A (MRTFA) transcription coactivator, it controls the expression of genes regulating cytoskeleton development, morphogenesis, and cell migration. It is also required for cardiac differentiation and maturation. SRF is shown to be activated by a variety of agents, including serum, lysophosphatidic acid, lipopolysaccharide, 12-O-tetradecanoylphorbol-13-acetate (TPA), and cytokines. Keratinocytes in normal human and mouse skin are reported to express high levels of SRF, but its expression is strongly downregulated in the hyperproliferative epidermis of wounded and psoriatic skin. Loss of SRF is known to disrupt epidermal homeostasis. Mice lacking SRF in cardiac tissue display lethal cardiac defects between E10.5 and E13.5. (Ref.: Hu, X., et al. (2019). Nat. Commun. 10(1); 1695; Koegel, H., et al. (2009). J. Clin. Invest. 119(4); 899-910; Parlakian , A., et al (2004). Mol. Cell Biol. 24(12); 5281-5289; Chai, J., and Tarnawski, AS. (2002). J. Physiol. Pharmacol. 53(2); 147-157).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SRF, clone 2C5, Cat. No. MABF3074, is a rat monoclonal antibody that detects serum response factor (SRF) and is used in Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected SRF in Immunofluorescence applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

      Immunohistochemistry Applications: A representative lot detected SRF in Immunohistochemistry applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

      Western Blotting Analysis: A representative lot detected SRF in Western Blotting applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

      Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected SRF in Chromatin Immunoprecipitation applications (Hu, X., et al. (2019). Nat Commun. 10(1):1695).

      Immunoprecipitation Analysis: A representative lot detected SRF in Immunoprecipitation applications (Hu, X., et al. (2019). Nat Commun. 10(1):1695).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenBacterially overexpressed His-tagged murine Serum response factor.
      EpitopeUnknown
      Clone2C5
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 2C5 is a rat monoclonal antibody that detects murine Serum response factor (SRF).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Srf
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight51.25 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human psoriatic skin tissue sections.

      Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:50 dilution of this antibody detected Serum response factor (SRF) in human psoriatic skin tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3074-100UG 04065268041994