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AB1761-I Anti-Protein Gene Product 9.5 Antibody

Anti-Protein Gene Product 9.5, Cat. No. AB1761-I is a rabbit polyclonal antibody that detects Protein Gene Product 9.5 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-Protein Gene Product 9.5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: UCHL1 UniProt Number: P09936
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AB1761-I
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWB, IH(P), IF, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1761-I
      ReplacesAB1761 and AB9724
      DescriptionAnti-Protein Gene Product 9.5 Antibody
      Alternate Names
      • Ubiquitin carboxyl-terminal hydrolase isozyme L1
      • UCH-L1
      • Neuron cytoplasmic protein 9.5
      • PGP 9.5
      • PGP9.5
      • Ubiquitin thioesterase L1
      Background InformationUbiquitin carboxyl-terminal hydrolase isozyme L1 (UniProt: P09936; also known as EC: 3.4.19.12, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1) is encoded by the UCHL1 gene (Gene ID: 7345) in human. UCH-L1 is a member of the peptidase C12 family that can exist as a monomer or homodimer. It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors and is detected in neuronal cell bodies and processes throughout the neocortex. It displays a weak expression in ovary. About 30% of total UCH-L1 is reported to be associated with membranes in brain. Its levels are shown to be down regulated in brains from Parkinson disease and Alzheimer disease patients. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin and is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimeric form may have ATP-independent ubiquitin ligase activity. Mutations in UCHL1 gene are known to cause Parkinson disease 5, a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Some mutations in this gene are also linked to spastic paraplegia 79, where patients display a form of spastic paraplegia, which is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH7.4 with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Protein Gene Product 9.5, Cat. No. AB1761-I is a rabbit polyclonal antibody that detects Protein Gene Product 9.5 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:20,000 dilution from a representative lot detected Protein Gene Product 9.5 in human and mouse brain tissue lysates.

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Protein Gene Product 9.5 in DU145 and E18 cortical cells.

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Protein Gene Product 9.5 in human pancreas and human cerebellum tissue sections.

      Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Protein Gene Product 9.5 in human cerebellum tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Protein Gene Product 9.5 (UCH-L1).
      EpitopeN-terminal
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Protein Gene Product 9.5 (UCH-L1) in multiple species. It targets and epitope within 18 amino acids from the N-terminal region.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UCHL1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~24 kDa observed; 24.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat Brain tissue lysate.

      Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Protein Gene Product 9.5 in Rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB1761-I 04053252929724

      Documentation

      Anti-Protein Gene Product 9.5 Antibody MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Protein Gene Product 9.5 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Protein Gene Product 9.5 2472051
      Anti-Protein Gene Product 9.5 - 2426669 2426669
      Anti-Protein Gene Product 9.5 - 2467132 2467132
      Anti-Protein Gene Product 9.5 - 2343843 2343843
      Anti-Protein Gene Product 9.5 - 2984135 2984135
      Anti-Protein Gene Product 9.5 - 3270756 3270756
      Anti-Protein Gene Product 9.5 - 3283215 3283215
      Anti-Protein Gene Product 9.5 - 3307787 3307787
      Anti-Protein Gene Product 9.5 - 3308248 3308248
      Anti-Protein Gene Product 9.5 - 3319469 3319469
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies