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DR1037 Anti-Plk1 Mouse mAb (35-206)

Anti-Plk1 Mouse mAb (35-206) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Polo-Like Kinase

DR1037
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~62 kDa Plk1 protein in HeLa cells.
      Catalogue NumberDR1037
      Brand Family Calbiochem®
      SynonymsAnti-Polo-Like Kinase
      Application Data
      Detection of human Plk1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells treated with nocodazole (100 ng/ml). Primary antibody: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (1 µg/ml). Detection: chemiluminescence.

      Detection of human Plk1 by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (446 µg) from HeLa cells treated with nocodazole (100 ng/ml). Antibody for IP: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (5 µg). Conditions for immunoblotting: same as above.
      References
      ReferencesTakai, N., et al. 2005. Oncogene 24, 287.
      Toyoshima-Morimoto, F., et al. 2002. EMBO Rep. 4, 341.
      Smits, V.A., et al. 2000. Nat. Cell Biol. 2, 672.
      Glover, D.M., et al. 1998. Genes Devel. 12, 3777.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlNocodazole-treated HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunoprecipitation (10 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenfull-length, recombinant, human Plk1
      ImmunogenHuman
      Clone35-206
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1037 0

      Documentation

      Anti-Plk1 Mouse mAb (35-206) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Plk1 Mouse mAb (35-206) Certificates of Analysis

      TitleLot Number
      DR1037

      References

      Reference overview
      Takai, N., et al. 2005. Oncogene 24, 287.
      Toyoshima-Morimoto, F., et al. 2002. EMBO Rep. 4, 341.
      Smits, V.A., et al. 2000. Nat. Cell Biol. 2, 672.
      Glover, D.M., et al. 1998. Genes Devel. 12, 3777.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      SynonymsAnti-Polo-Like Kinase
      ApplicationImmunoblotting (1 µg/ml)
      Immunoprecipitation (10 µg/ml)
      Application Data
      Detection of human Plk1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells treated with nocodazole (100 ng/ml). Primary antibody: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (1 µg/ml). Detection: chemiluminescence.

      Detection of human Plk1 by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (446 µg) from HeLa cells treated with nocodazole (100 ng/ml). Antibody for IP: Anti-Plk1 Mouse mAb (35-206) (Cat. No. DR1037) (5 µg). Conditions for immunoblotting: same as above.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~62 kDa Plk1 protein.
      BackgroundPlk1 (ACC# NP_005021) is an evolutionarily conserved member of the polo-like serine/threonine kinase family that plays a critical role in spindle formation, chromosome segregation, regulation of the anaphase-promoting complex (APC), and execution of cytokinesis. Predominantly cytoplasmic during interphase, Plk1 is associated with condensed chromatin during mitosis when its kinase activity peaks. Plk1 regulates mitotic events through phosphorylation of its substrates Cdc25, the activator of Cdc2, and Cdc27, a component of the APC. Phosphorylation of Cdc25C by Plk1 is thought to lead to nuclear accumulation of Cdc25 and activation of Cdc2. Phosphorylation of Cdc27 leads to destruction of the APC. Ectopic expression of Plk1 in NIH3T3 leads to transformation. Over-expression of Plk1 is positively correlated with aggressiveness and prognosis of many cancers and inhibition of Plk1 activity results in growth arrest and apoptosis in cancer cells. Plk1 has been shown to be elevated in numerous cancers including lung, breast, ovarian, melanoma, colorectal, and gliomas.
      HostMouse
      Immunogen speciesHuman
      Immunogenfull-length, recombinant, human Plk1
      Clone35-206
      IsotypeIgG2b
      Specieshuman, mouse, rat
      Positive controlNocodazole-treated HeLa cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesTakai, N., et al. 2005. Oncogene 24, 287.
      Toyoshima-Morimoto, F., et al. 2002. EMBO Rep. 4, 341.
      Smits, V.A., et al. 2000. Nat. Cell Biol. 2, 672.
      Glover, D.M., et al. 1998. Genes Devel. 12, 3777.