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PC21 Anti-PDGF (Ab-1) (101-116) Rabbit pAb

This Anti-PDGF (Ab-1) (101-116) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections, ELISA for the detection of PDGF (Ab-1) (101-116).

Anti-PDGF (Ab-1) (101-116) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Platelet Derived Growth Factor

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PC21
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the B subunit of PDGF in mesenchymal cells and fetal epithelial tissue.
      Catalogue NumberPC21
      Brand Family Calbiochem®
      SynonymsAnti-Platelet Derived Growth Factor
      References
      ReferencesLaRochelle, W.J., et al. 1989 Mol. Cell. Biol. 9, 3538.
      Hannink, M., et al. 1986 Mol. Cell. Biol. 6, 1304.
      Ross, R., et al. 1986 Cell 46, 155.
      Devare, S.G., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 731.
      Waterfield, M.D., et al. 1983. Nature (London) 304, 35.
      Johnsson, A., et al. 1982 Biochem. Biophys. Res. Commun. 104, 66.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlPC12 cells
      Positive controlMesenchymal cells or fetal epithelial tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Paraffin Sections
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, heat pre-treatment required)
      ELISA (see comments)
      Application CommentsThis antibody specific for the B form of human PDGF that will react by immunofluorescence with human platelets and has been reported to react in ELISA with the PDGF(AB) and PDGF(BB) protein. Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogena synthetic peptide (TEVFEISRRLIDRTNA) corresponding amino acids 101-116 of human PDGF B chain
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC21 0

      Documentation

      Anti-PDGF (Ab-1) (101-116) Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PDGF (Ab-1) (101-116) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC21

      References

      Reference overview
      LaRochelle, W.J., et al. 1989 Mol. Cell. Biol. 9, 3538.
      Hannink, M., et al. 1986 Mol. Cell. Biol. 6, 1304.
      Ross, R., et al. 1986 Cell 46, 155.
      Devare, S.G., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 731.
      Waterfield, M.D., et al. 1983. Nature (London) 304, 35.
      Johnsson, A., et al. 1982 Biochem. Biophys. Res. Commun. 104, 66.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      SynonymsAnti-Platelet Derived Growth Factor
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, heat pre-treatment required)
      ELISA (see comments)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the B form of human PDGF.
      BackgroundPlatelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The protein may exist as either a heterodimer AB or as a homodimer AA or BB. PDGF (BB) has been identified as the human homolog of the v-sis oncogene product and as such has been designated c-sis. The v-sis transforming domain is 89 amino acids in length and is identical in sequence to PDGF (BB). Binding of the PDGF molecule to its specific receptor occurs via interaction with a specific domain on the surface of the molecule known as the V4 surface domain. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (TEVFEISRRLIDRTNA) corresponding amino acids 101-116 of human PDGF B chain
      IsotypeIgG
      Specieshuman, rat
      Positive controlMesenchymal cells or fetal epithelial tissue
      Negative controlPC12 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody specific for the B form of human PDGF that will react by immunofluorescence with human platelets and has been reported to react in ELISA with the PDGF(AB) and PDGF(BB) protein. Antibody should be titrated for optimal results in individual sample types.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesLaRochelle, W.J., et al. 1989 Mol. Cell. Biol. 9, 3538.
      Hannink, M., et al. 1986 Mol. Cell. Biol. 6, 1304.
      Ross, R., et al. 1986 Cell 46, 155.
      Devare, S.G., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 731.
      Waterfield, M.D., et al. 1983. Nature (London) 304, 35.
      Johnsson, A., et al. 1982 Biochem. Biophys. Res. Commun. 104, 66.