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PC364 Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb

Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC364
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~70 kDa netrin-1 protein in mouse brain tissue. Supplied with a control peptide.
      Catalogue NumberPC364
      Brand Family Calbiochem®
      Application Data
      Detection of mouse netrin-1 by staining frozen sections. Sample: Day 14 embryonic mouse brain tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb (Cat. No. PC364) (2 μg/ml).
      References
      ReferencesVarela-Echavarria, A. and Guthrie, S. 1997. Genes and Dev. 11, 545.
      Harris, R., et al. 1996. Neuron 17, 217.
      Keino-Masu, K., et al. 1996. Cell 87, 175.
      Mitchell, K.J., et al. 1996. Neuron 17, 203.
      Serafini, T., et al. 1996. Cell 87, 1001.
      Tessier-Lavigne, M. and Goodman, C.S., 1996. Science 274, 1123.
      Kennedy, T.E., et al. 1994. Cell 78, 425.
      Serafini, T., et al. 1994. Cell 78, 409.
      Ishii, N., et al. 1992. Neuron 9, 873.
      Hedgecock, E.M., et al. 1990. Neuron 4, 61.
      Product Information
      FormLiquid
      Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide (immunogen).
      Positive controlMouse brain tissue
      Preservative≤0.1% sodium azide (antibody only)
      Applications
      Key Applications Frozen Sections
      Not Western Blot
      Application NotesFrozen Sections (1-2 µg/ml)
      Immunoblotting (not recommended)
      Application CommentsNetrin-1 is enriched in embryonic neural tissue. Immunohistochemistry was performed using embryonic day 14 (E14) mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with control peptide at 10-6M. Unconjugated Netrin-1 Control Peptide has been included for use as an internal control for antibody specificity. For immunoblotting applications, we recommend Netrin-1 (Ab-1) Polyclonal Antibody (Cat. No. PC344). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (QIHILKADKAGD) corresponding to amino acids 497-508 of mouse netrin-1, conjugated to KLH
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsStore lyophilized peptide at -20°C. Reconstitute peptide with 25 µl of dH₂O for a working stock of 1 mg/ml. Following reconstitution of peptide, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC364 0

      Documentation

      Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC364

      References

      Reference overview
      Varela-Echavarria, A. and Guthrie, S. 1997. Genes and Dev. 11, 545.
      Harris, R., et al. 1996. Neuron 17, 217.
      Keino-Masu, K., et al. 1996. Cell 87, 175.
      Mitchell, K.J., et al. 1996. Neuron 17, 203.
      Serafini, T., et al. 1996. Cell 87, 1001.
      Tessier-Lavigne, M. and Goodman, C.S., 1996. Science 274, 1123.
      Kennedy, T.E., et al. 1994. Cell 78, 425.
      Serafini, T., et al. 1994. Cell 78, 409.
      Ishii, N., et al. 1992. Neuron 9, 873.
      Hedgecock, E.M., et al. 1990. Neuron 4, 61.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      ApplicationFrozen Sections (1-2 µg/ml)
      Immunoblotting (not recommended)
      Application Data
      Detection of mouse netrin-1 by staining frozen sections. Sample: Day 14 embryonic mouse brain tissue fixed with 4% paraformaldehyde. Primary antibody: Anti-Netrin-1 (Ab-2) (497-508) Rabbit pAb (Cat. No. PC364) (2 μg/ml).
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~70 kDa netrin-1 protein. Supplied with a control peptide (immunogen).
      BackgroundIn the developing nervous system, axon guidance and the establishment of appropriate neuronal connections involves a number of chemoattractant and chemorepellent molecules. Netrins are related to the extracellular matrix protein laminin and comprise a family of secreted molecules capable of guiding the growth cone. The first netrin family member to be characterized, UNC-6 from C. elegans, was initially identified from the gene mutation which lead to defects in circumferential axon migration in the nematode. In contrast, the identification of the first vertebrate netrins, designated netrin-1 and netrin-2, resulted from a biochemical rather than genetic approach. Netrin homologs have subsequently been identified from a number of species. Results from several studies suggest a role for netrin-1 in mediating chemotropic and outgrowth-promoting activities of floor plate cells to guide commissural axons towards the ventral midline. DCC (Deleted in Colorectal Cancer) is believed to function as a component of a receptor complex which mediates the effects of netrin-1 on commissural axons and helps coordinate the navigation of axons to establish precise patterns of neural connectivity. In addition to the netrins and their receptors, a number of other diffusable or membrane-associated ligand families and their receptors have been implicated in axon guidance, including the semaphorins, ephrins, and cell adhesion molecules.
      HostRabbit
      Immunogen speciesMouse
      Immunogena synthetic peptide (QIHILKADKAGD) corresponding to amino acids 497-508 of mouse netrin-1, conjugated to KLH
      IsotypeIgG
      Speciesmouse
      Positive controlMouse brain tissue
      FormLiquid
      Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide (immunogen).
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide (antibody only)
      CommentsNetrin-1 is enriched in embryonic neural tissue. Immunohistochemistry was performed using embryonic day 14 (E14) mouse brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with control peptide at 10-6M. Unconjugated Netrin-1 Control Peptide has been included for use as an internal control for antibody specificity. For immunoblotting applications, we recommend Netrin-1 (Ab-1) Polyclonal Antibody (Cat. No. PC344). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsStore lyophilized peptide at -20°C. Reconstitute peptide with 25 µl of dH₂O for a working stock of 1 mg/ml. Following reconstitution of peptide, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesVarela-Echavarria, A. and Guthrie, S. 1997. Genes and Dev. 11, 545.
      Harris, R., et al. 1996. Neuron 17, 217.
      Keino-Masu, K., et al. 1996. Cell 87, 175.
      Mitchell, K.J., et al. 1996. Neuron 17, 203.
      Serafini, T., et al. 1996. Cell 87, 1001.
      Tessier-Lavigne, M. and Goodman, C.S., 1996. Science 274, 1123.
      Kennedy, T.E., et al. 1994. Cell 78, 425.
      Serafini, T., et al. 1994. Cell 78, 409.
      Ishii, N., et al. 1992. Neuron 9, 873.
      Hedgecock, E.M., et al. 1990. Neuron 4, 61.