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AB2963-25UG Anti-MMP-3 Antibody, N-terminus

Detect MMP-3 using this Anti-MMP-3 Antibody, N-terminus validated for use in WB, IH(P).

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AB2963-25UG
25 μg  
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      Description
      Catalogue NumberAB2963-25UG
      DescriptionAnti-MMP-3 Antibody, N-terminus
      Alternate Names
      • matrix metallopeptidase 3 (stromelysin 1, progelatinase)
      • matrix metalloproteinase 3 (stromelysin 1, progelatinase)
      • Matrix metalloproteinase-3
      • transin-1
      • proteoglycanase
      • stromelysin-1
      • Transin-13
      Background InformationMMP-3 (matrix metalloproteinase-3), also known as stromelysin-1, is a member of the matrix metalloproteinase (MMP) family of zinc endopeptidases. MMP’s are required for removal of extracellular matrix machinery during embryo development, morphogenesis and tissue remodeling. Endogenous tissue inhibitors of metalloproteinases (TIMPs) are responsible for regulating MMP-3. Any disruption of this system can cause diseases such as arthritis, metastasis, atherosclerosis and tumor growth.
      References
      Product Information
      FormatAffinity Purified
      Control
      • PMA-treated HL-60 concentrated media
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect MMP-3 using this Anti-MMP-3 Antibody, N-terminus validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:600 dilution from a previous lot detected MMP-3 in colorectal carcinoma and ductal carcinoma tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human N-terminal MMP-3.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody recognizes MMP-3 at the N-terminus.
      Species Reactivity
      • Human
      • Chimpanzee
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with human.
      Predicted to react with chimpanzee and rhesus macaque based on 100% sequence homology.
      Other homologies: Mouse and rat (64% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq].
      Gene Symbol
      • MMP3
      • STMY
      • STMY1
      • SL-1
      • MMP-3
      • CHDS6
      • STR1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

      CATALYTIC ACTIVITY: Preferential cleavage where P1', P2' and P3' are hydrophobic residues.

      COFACTOR: Binds 4 calcium ions per subunit.

      Binds 2 zinc ions per subunit.

      SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix Probable.

      DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

      SEQUENCE SIMILARITIES: Belongs to the peptidase M10A family.

      Contains 4 hemopexin-like domains.
      Molecular Weight~58 kDa observed.
      An uncharacterized band may be seen at ~78 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PMA-treated HL-60 concentrated media.

      Western Blot Analysis: 0.1-1 µg/mL of this antibody detects MMP-3 in 10 µg of PMA-treated HL-60 concentrated media.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB2963-25UG 04054839342561

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