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428017 Anti-Lamp1 Mouse mAb (LY1C6)

This Anti-Lamp1 Mouse mAb (LY1C6) is validated for use in Immunoblotting, Immunocytochemistry, Immunofluorescence, Immunoprecipitation for the detection of Lamp1.

Anti-Lamp1 Mouse mAb (LY1C6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Lysosome-Associated Membrane Protein 1

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428017
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
RMMonoclonal Antibody

Pricing & Availability

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428017-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~120 kDa Lamp1 protein.
      Catalogue Number428017
      Brand Family Calbiochem®
      SynonymsAnti-Lysosome-Associated Membrane Protein 1
      Application Data
      Detection of hamster Lamp1 by immunocytochemistry. Sample: CHO K1 cells. Primary antibody: Anti-Lamp1 Mouse mAb (LY1C6) (Cat. No. 428017) (1:100). Detection: fluorescence.
      References
      ReferencesKannan, K. et al. 1996. Cell Immunol. 171, 10.
      Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
      Howe et al. 1988. PNAS 85, 7577.
      Lewis et al. 1985. J. Cell Biol. 100, 1839.
      Product Information
      FormLiquid
      FormulationIn PBS containing 50% glycerol, pH 7.2.
      Positive controlCHO-K1 cells
      Preservative≤0.09% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
      Immunocytochemistry (1:100)
      Immunofluorescence (see comments)
      Immunoprecipitation (see comments)
      Application CommentsThis antibody has also been reported to work for immunofluorescence and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrat liver lysosomal membranes
      ImmunogenRat
      CloneLY1C6
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      428017-100UG 04055977210422

      Documentation

      Anti-Lamp1 Mouse mAb (LY1C6) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Lamp1 Mouse mAb (LY1C6) Certificates of Analysis

      TitleLot Number
      428017

      References

      Reference overview
      Kannan, K. et al. 1996. Cell Immunol. 171, 10.
      Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
      Howe et al. 1988. PNAS 85, 7577.
      Lewis et al. 1985. J. Cell Biol. 100, 1839.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-June-2016 RB
      SynonymsAnti-Lysosome-Associated Membrane Protein 1
      ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
      Immunocytochemistry (1:100)
      Immunofluorescence (see comments)
      Immunoprecipitation (see comments)
      Application Data
      Detection of hamster Lamp1 by immunocytochemistry. Sample: CHO K1 cells. Primary antibody: Anti-Lamp1 Mouse mAb (LY1C6) (Cat. No. 428017) (1:100). Detection: fluorescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~120 kDa Lamp1 protein.
      BackgroundLyso-some-associated membrane protein (Lamp1), also known as lgpA, is a type 1 integral membrane protein that is transported from the trans-Golgi net-work to endosomes and then lysosomes. Upon cell activation, Lamp1 is transferred to the plasma membrane. Cell surface Lamp1 and Lamp2 promote adhesion of peripheral blood mononuclear cells (PBMC) to vascular endothelium and may be involved in the adhesion of PBMC to the site of inflammation.
      HostMouse
      Immunogen speciesRat
      Immunogenrat liver lysosomal membranes
      CloneLY1C6
      IsotypeIgG₁
      Speciesrat
      Positive controlCHO-K1 cells
      FormLiquid
      FormulationIn PBS containing 50% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.09% sodium azide
      CommentsThis antibody has also been reported to work for immunofluorescence and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKannan, K. et al. 1996. Cell Immunol. 171, 10.
      Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
      Howe et al. 1988. PNAS 85, 7577.
      Lewis et al. 1985. J. Cell Biol. 100, 1839.