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MABN2268-25UG Anti-LRRK1 Antibody, clone 12F2.1

Anti-LRRK1, clone 12F2.1, Cat. No. MABN2268, is a highly specific mouse monoclonal antibody that detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category: Neuroscience Gene Symbol: LRRK1, KIAA790 UniProt Number: Q38SD2
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MABN2268-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2268-25UG
      DescriptionAnti-LRRK1 Antibody, clone 12F2.1
      Alternate Names
      • Leucine-rich repeat serine/threonine-protein kinase 1
      • Leucine-rich repeat kinase 1
      Background InformationLeucine-rich repeat serine/threonine-protein kinase 1 (UniProt: Q38SD2, Leucine-rich repeat kinase 1) is encoded by the LRRK1 (also known as KIAA1790) gene (Gene ID: 79705) in human. Leucine-rich repeat kinase 1 is a member of the ROCO family of protein kinases. Two isoforms of Leucine-rich repeat kinase 1 have been described that are generated by alternative splicing. It contains a ROC (Ras in complex proteins) domain together with a MAPKKK-like kinase domain and several protein protein interactions. It is reported to form a complex with activated EGFR via interaction with Grb2. Subsequently, it is internalized to early endosomes along with EGFR. Leucine-rich repeat kinase 1 also regulates EGFR transport from early to late endosomes and is involved in regulating the motility of EGF-containing early endosomes that is dependent on its kinase activity. Leucine-rich repeat kinase 1 is also reported to play a role in regulating bone mass and mice with defective gene are prone to osteoporosis, increased bone mineralization, and diminished bone resorption. (Ref.: Hanafusa, H et al. (2011). Nature Commun. 2, Article number: 158).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-LRRK1, clone 12F2.1, Cat. No. MABN2268, is a highly specific mouse monoclonal antibody that detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected LRRK1 in human cerebral cortex, human testis, and human pancreatic cancer tissues.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 86 amino acids from the internal region of human Leucine-rich repeat serine/threonine-protein kinase 1.
      Clone12F2.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 12F2.1 detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) in human. It targets an epitope within 86 amino acids from the internal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • LRRK1
      • KIAA790
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~200 kDa observed; 225.39 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human hypothalamus tissue lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected LRRK1 in 10 µg of human hypothalamus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2268-25UG 04054839522093

      Documentation