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MABS2066-25UG Anti-LLGL1 Antibody, clone mAb17-35

Anti-LLGL1, clone mAb17-35, Cat. No. MABS2066, is a mouse monoclonal antibody that detects Lethal(2) giant larvae protein homolog 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Anti-LLGL1 Antibody, clone mAb17-35 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Llgl1, Llglh UniProt Number: Q80Y17
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MABS2066-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2066-25UG
      DescriptionAnti-LLGL1 Antibody, clone mAb17-35
      Alternate Names
      • Lethal(2) giant larvae protein homolog 1
      • Mgl-1
      • Mlgl
      Background InformationLethal(2) giant larvae protein homolog 1 (UniProt: Q80Y17; also known as LLGL, Mgl-1, Mlgl) is encoded by the Llgl1 (also known as Llglh) gene (Gene ID: 16897) in murine species. LLGL belongs to the WD repeat L(2)GL family. LLGL is a cortical cytoskeleton protein that is found in a complex involved in maintenance of cell polarity and epithelial integrity. It is involved in the regulation of mitotic spindle orientation, proliferation, differentiation, and tissue organization of neuroepithelial cells. It is generally enriched in developing axons. LLGL stability and function is achieve by its interaction with RanBPM protein, which primarily acts as a scaffolding protein. Its N-terminal region containing SPRY domain is shown to be responsible for binding to RanBPM and this binding functionally extends its half-life by preventing its turnover through ubiquitin-proteasomal pathway. RanBPM is shown enhance tumor suppressor activity of LLGL by inhibiting LLGL-mediated cell proliferation and cell migration. (Ref.: Suresh, B., et al. (2010). J. Biol. Chem. 285(46); 35340-35349).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-LLGL1, clone mAb17-35, Cat. No. MABS2066, is a mouse monoclonal antibody that detects Lethal(2) giant larvae protein homolog 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected LLGL1 in Western Blotting applications (Ivanov, A.I., et. al. (2010). Am J Pathol. 176(1):134-45).

      Immunocytochemistry Analysis: A representative lot detected LLGL1 in Immunocytochemistry applications (Ivanov, A.I., et. al. (2010). Am J Pathol. 176(1):134-45).

      Immunohistochemistry Analysis: A representative lot detected LLGL1 in Immunohistochemistry applications (Weimer, J.M., et. al. (2009). Development. 136(17):2965-75).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 153 amino acids from the C-terminal region of murine Lethal(2) giant larvae protein homolog 1 (LLGL).
      EpitopeC-terminus
      ClonemAb17-35
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone mAb17-35 specifically detects LLGL1 in mouse. It targets an epitope with in 153 amino acids from the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Llgl1
      • Llglh
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~120 kDa observed; 112.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 4 µg/mL of this antibody detected LLGL1 in mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2066-25UG 04054839563256