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MABC1210 Anti-Hox-C10 Antibody, clone 2F11.1

This Anti-Hox-C10 Antibody, clone 2F11.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of Hox-C10.

Anti-Hox-C10 Antibody, clone 2F11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Transcription Factors Gene Symbol: HOXC10, HOX3I UniProt Number: Q9NYD6
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MABC1210
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IH(P), ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1210
      DescriptionAnti-Hox-C10 Antibody, clone 2F11.1
      Alternate Names
      • Homeobox protein Hox-C10
      • Homeobox protein Hox-3I
      Background InformationHomeobox protein Hox-C10 (UniProt Q9NYD6; also known as Homeobox protein Hox-3I) is encoded by the HOXC10 (also known as HOX3I) gene (Gene ID 3226) in human. HOXC10 belongs to a family of highly conserved homeobox transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, with HOXC genes located in a cluster on chromosome 12. Hox-C10 is also implicated to be involved in replication, where it binds in a structure-dependent manner to origins of replication and contributes to the assembly of replicative complexes. HOXC10 transcription is reported to be silenced in two estrogen deprivation-resistent cell lines, C4-12 and LTED, derived from MCF-7 breast cancer cells as a result of epigenetic hypermethylation as well as additional repressive histone modifications. Evidence indicates that Hox-C10 expression is repressed by estrogen in vitro and in vivo to promote tumor growth. Short-term estrogen withdrawal restores Hox-OXC10 expression to induce growth arrest and apoptosis, but its long-term absence results in permanent repression as a result of epigenetic methylation and the development of estrogen-independent cancer cells.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Hox-C10 Antibody, clone 2F11.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of Hox-C10.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Hox-C10 in A431, HeLa, HepG2, and NIH/3T3 cells.
      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Hox-C10 in human colon cancer tissue, as well as in normal kidney and panreas tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human Hox-C10 internal fragment.
      EpitopeInternal.
      Clone2F11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2F11.1 recognizes an internal epitope of human homeobox protein Hox-C10, also known as Hox-3I.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HOXC10
      • HOX3I
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~38 kDa observed. 38.07 kDa calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Hox-C10 in 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1210 04055977353587