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MAB4212F Anti-HU-B27 Antibody, FITC conjugated

Detect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB.

Anti-HU-B27 Antibody, FITC conjugated MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: MRAP, C21orf61, FGD2, FALP, B27, GCCD2 UniProt Number: P03989
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MAB4212F
1 mL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, ICC, IF, WBMFITCMonoclonal Antibody
      Description
      Catalogue NumberMAB4212F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-HU-B27 Antibody, FITC conjugated
      Alternate Names
      • Com-B27
      Background InformationHU-B27 (HLA- Com-B27) is involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
      References
      Product Information
      FormatFITC
      Control
      • Control IgG2a-FITC (MABC002F) as an isotype control
      PresentationThe reagent is supplied in 1.0 mL phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. Purified from mouse ascitic fluid. The characteristics of each lot are tested by gel electrophoresis and flow cytometry.
      Quality LevelMQ100
      Applications
      ApplicationDetect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesFlow cytometry and Immunofluorescence: use 10 mL direct from the vial per 100 mL of whole blood, or 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 mL buffer.

      Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
      Subjects/Fluorescence Intensity
      Heterozygous HLA-B27+/Strong
      Homozygous HLA-B7+/Weak
      Heterozygous HLA-B7+/Weak - negative
      Non B27, Non B7/Negative

      This reagent is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis.

      SUGGESTED USAGE:
      Flow cytometry and immunofluorescence - use 10 µl direct from the vial per 100 µl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µl buffer.

      Use Control IgG2a-FITC (MABC002F) as an isotype control.

      Please note that this reagent should be used in a single-colour single-step assay only.
      Biological Information
      HostMouse
      SpecificityThis reagent binds strongly to the HLA-B27 antigen. It also binds weakly to the HLAB7 antigen. This formulation demonstrates less cross reactivity to HLA-B7 than Chemicon products, MAB1285 and MAB1285F.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MRAP
      • C21orf61
      • FGD2
      • FALP
      • B27
      • GCCD2
      UniProt Number
      Molecular Weight19 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Jurkat lysates.

      Western Blotting Analysis:
      1:500 dilution of this antibody detected Com-B27 on 10 µg of Jurkat lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC in the dark from date of receipt.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4212F 04053252317200

      Documentation

      Anti-HU-B27 Antibody, FITC conjugated MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-HU-B27 Antibody, FITC conjugated Certificates of Analysis

      TitleLot Number
      Anti-HU-B27, FITC conjugated 2470854
      Anti-HU-B27, FITC conjugated - 2383541 2383541
      Anti-HU-B27, FITC conjugated - 2430402 2430402
      Anti-HU-B27, FITC conjugated - 2441930 2441930
      Anti-HU-B27, FITC conjugated - 08051488828 08051488828
      Anti-HU-B27, FITC conjugated - 08061492515 08061492515
      Anti-HU-B27, FITC conjugated - 2109523 2109523
      Anti-HU-B27, FITC conjugated - 2118021 2118021
      Anti-HU-B27, FITC conjugated - 2295666 2295666
      Anti-HU-B27, FITC conjugated - 2328355 2328355
      Show more

      References

      Reference overviewPub Med ID
      Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
      Trapani, J A, et al.
      Hum. Immunol., 7: 205-16 (1983)  1983

      Show Abstract       		6604046 6604046

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      Alternative Packsize

      Catalogue Number Description  
      MAB4212F-K Anti-Hu-B27, FITC conjugated (1mL) Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies