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MABF2350-25UG Anti-GSDMD Antibody, clone 8C2

Anti-GSDMD, clone 8C2, Cat. No. MABF2350, is a mouse monoclonal antibody that detects Gasdermin-D and is tested for use in Immunocytochemistry and Western Blotting.

MABF2350-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2350-25UG
      DescriptionAnti-GSDMD Antibody, clone 8C2
      Alternate Names
      • Gasdermin-D
      Background InformationGasdermin-D (UniProt: P57764; also known as Gasdermin domain-containing protein 1) is encoded by the GSDMD (also known as DFNA5L, GSDMDC1, FKSG10) gene (Gene ID: 79792) in human. Gasdermin-D is a member of the gasdermin family of proteins that promotes inflammatory cell death (pyroptosis) in response to microbial infection and danger signals. Its expression is observed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach. Gasdermin-2 consists of a 31 kDa N-terminal and a 22 kDa C-terminal domains that are separated by a linker region. Under normal conditions, the full-length Gasdermin-D is inactive as the linker loop between the N-terminal and C-terminal domains stabilizes the overall conformation of the full-length protein and allows the C-terminal domain to fold back on and auto-inhibit the N-terminal Gasdermin-D (Gasdermin-N). Upon interdomain cleavage by the action of caspase-1 and 4 at Asp275, the autoinhibition is relieved and Gasdermin-N cytotoxicity is triggered. Gasdermin-N is reported to form membrane pore that lead to cytokine release and pyroptosis. It also displays bactericidal activity. Gasdermin-N released from pyroptotic cells into the extracellular milieu can bind to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells. (Ref.: Liu, X., et al. (2016). Nature. 535(7610); 153-158; Hu, JJ., et al. (2020). Nat. Immunol. 21(7):736-745).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-GSDMD, clone 8C2, Cat. No. MABF2350, is a mouse monoclonal antibody that detects Gasdermin-D and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected recombinant Gasdermin-D (Courtesy of Judy Lieberman, M.D., Ph.D. and Zhibin Zhang, Ph.D., Boston Children's Hospital/Harvard Medical School, Massachusetts, USA).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length recombinant human Gasdermin-D.
      EpitopeUnknown
      Clone8C2
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 8C2 is a mouse monoclonal antibody that detects human Gasdermin-D.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GSDMD
      • DFNA5L
      • GSDMDC1
      • FKSG10
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight52.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HEK293T cells .

      Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Gasdermin-D in HEK293T cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2350-25UG 04065265324311