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ST1629 Anti-GNA13 Mouse mAb (6F6-B5)

Anti-GNA13, mouse monoclonal, clone 6F6-B5, recognizes the ~40-45 kDa GNA13 protein in HepG2 cells. It is validated for use in ELISA and Western blotting.

Anti-GNA13 Mouse mAb (6F6-B5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13

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ST1629
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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ST1629-100UG
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      Description
      OverviewRecognizes the ~40-45 kDa GNA13 protein in HepG2 cells.
      Catalogue NumberST1629
      Brand Family Calbiochem®
      SynonymsAnti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13
      Application Data
      Detection of human GNA13 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-GNA13 Mouse mAb (6F6-B5) (Cat. No. ST1629) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHepG2 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human GNA13 (aa 1-378), expressed as a GST fusion protein
      Clone6F6-B5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1629-100UG 04055977223729

      Documentation

      Anti-GNA13 Mouse mAb (6F6-B5) MSDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-November-2010 RFH
      SynonymsAnti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human GNA13 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-GNA13 Mouse mAb (6F6-B5) (Cat. No. ST1629) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~40-45 kDa GNA13 protein.
      BackgroundHeterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an α-, β- and γ-subunit and is bound to GDP in the 'off' state. Ligand binding to a 7-TM receptor causes a conformational change in the receptor, which detaches the G protein. This reaction switches the G protein to an 'on' state, allowing GTP to displace GDP, thus Gα can activate second messenger signaling cascades. There are several types of Gα proteins; Gαs and Gαi bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively. Gαq/11 stimulates PLCβ which catalyzes the hydroylsis of PIP2 to the second messengers IP3 and DAG. Gv12/13 are involved in Rho family GTPase signaling. In addition, some Gβγ subunits have active functions; Gβv coupled to H1 receptors can activate PLA2 and Gβγ coupled to M1 receptors can activate KIR channels. Rapid termination of the cellular response is required once the concentration of the stimulating ligand decreases. GTP bound to Gα is hydrolyzed rapidly to GDP, Gα is converted to its inactive form and re-associates with the β- and γ-subunits returning to the 'off' state.
      HostMouse
      ImmunogenFull-length, recombinant, human GNA13 (aa 1-378), expressed as a GST fusion protein
      Clone6F6-B5
      IsotypeIgG₁
      Specieshuman
      Positive controlHepG2 cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review