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DR1083 Anti-GCLM Mouse mAb (2B8)

Anti-GCLM Mouse mAb (2B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glutamate-Cysteine Ligase Modifier Subunit

DR1083
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~30 kDa GCLM protein in HeLa cells.
      Catalogue NumberDR1083
      Brand Family Calbiochem®
      SynonymsAnti-Glutamate-Cysteine Ligase Modifier Subunit
      Application Data
      Detection of human GCLM by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GCLM Mouse mAb (2B8) (Cat. No. DR1083) (1-5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesBalamurugan, A,N., et al. 2009. Pancreas 38, 454.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human GCLM (aa 1-275), expressed as a GST fusion protein
      Clone2B8
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1083 0

      Documentation

      References

      Reference overview
      Balamurugan, A,N., et al. 2009. Pancreas 38, 454.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-Glutamate-Cysteine Ligase Modifier Subunit
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human GCLM by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GCLM Mouse mAb (2B8) (Cat. No. DR1083) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~30 kDa GCLM protein.
      BackgroundGlutamate-cysteine ligase, also known as γ-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
      HostMouse
      ImmunogenFull-length, recombinant, human GCLM (aa 1-275), expressed as a GST fusion protein
      Clone2B8
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesBalamurugan, A,N., et al. 2009. Pancreas 38, 454.