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MABS1915-25UG Anti-EGFRvIII Antibody, clone DH8.3

Anti–EGFRvIII, clone DH8.3 Antibody, Cat. No. MABS1915-25UG, validated for use in Western Blotting, ELISA, Immunohistochemistry, Flow Cytometry and Immunoprecipitation.

Anti-EGFRvIII Antibody, clone DH8.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: EGFR, ERBB, ERBB1, HER1 UniProt Number: P00533
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MABS1915-25UG
25 μg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISA, IHC, FC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1915-25UG
      DescriptionAnti-EGFRvIII Antibody, clone DH8.3
      Alternate Names
      • Proto-oncogene c-ErbB-1
      • Receptor tyrosine-protein kinase erbB-1
      • Epidermal growth facttor receptor vIII
      Background InformationEpidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type 1 membrane protein that is ubiquitously expressed. It is synthesized with a 24 amino acids signal peptide that is subsequently cleaved. It contains an extracellular domain (aa 25-645) a transmembrane domain (aa 646-668), and a cytoplasmic domain (aa 669-1210). Upon binding of EGF, the receptor is phosphorylated and recruits adapter proteins to turn on downstream signaling cascades. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes. The most common truncated EGFR is the variant III EGFR deletion mutant (EGFRvIII), containing an inframe deletion of exons 2 7 (801 bp) from the extracellular domain. EGFRvIII mutation is reported in about 5% of human lung squamous cell carcinoma (SCC). It has also been suggested that EGFRvIII potentially plays an essential role in the human NSCLC tumor initiation and maintenance. Drugs such as Gefitinib, erlotnib, and HKI-272 are shown to inhibit the growth of EGFRvIII transformed cells and tumor growth. (Ref.: Ji, H., et al. (2006). Proc. Natl. Acad. Sci. USA 103(20); 7817-7822).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti–EGFRvIII, clone DH8.3 Antibody, Cat. No. MABS1915-25UG, validated for use in Western Blotting, ELISA, Immunohistochemistry, Flow Cytometry and Immunoprecipitation.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected EGFRvIII in Western Blotting applications (Lammering, G., et. al. (2004). Clin Cancer Res. 10(19):6732-43; Nishikawa, R., et. al. (2004). Brain Tumor Pathol. 21(2):53-6; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to EGFRvIII (EGFR variant III) (LEEKKGNYVVTDHC) sequence.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone DH8.3 detects EGFR variant vIII a truncated form of EGFR, present in human cancer cells.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • EGFR
      • ERBB
      • ERBB1
      • HER1
      Purification MethodProtein G purified
      UniProt Number
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U87MG.∆EGFR cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1915-25UG 04054839420986