MAB3199Z
Anti-E-Cadherin Antibody, clone 67A4, Azide Free
clone 67A4, Chemicon®, from mouse
Synonym(s):
CD324, MAB3199
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
67A4, monoclonal
Application:
FACS, IF
Citations:
10
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
67A4, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunofluorescence: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDH1(999)
Immunogen
T-47D breast carcinoma line.
Application
Anti-E-Cadherin Antibody, clone 67A4, Azide Free is an antibody against E-Cadherin for use in FC & IF.
Immunoblotting
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Optimal working dilutions must be determined by end user.
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.
Physical form
Format: Purified
Liquid in 0.02M PBS with NaCl, pH=7.6, with no preservatives. Protein A purified immunoglobulin.
Analysis Note
Tested
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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