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MABE644 Anti-Cre Recombinase Antibody, clone 5B4.1

Anti-Cre Recombinase Antibody, clone 5B4.1, Cat. No. MABE644, is a highly specific mouse monoclonal antibody that targets Cre recombinase and has been tested in Western Blotting.

Anti-Cre Recombinase Antibody, clone 5B4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: cre UniProt Number: P06956
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MABE644
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE644
      ReplacesMABE3120
      DescriptionAnti-Cre Recombinase Antibody, clone 5B4.1
      Alternate Names
      • Recombinase cre
      Background InformationRecombinase cre (UniProt P06956) is encoded by the cre gene (Gene ID 2777477) of bacteriophage P1. Cre (Causes recombination with Cyclization recombinase) is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each binds to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals vivo.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cre Recombinase Antibody, clone 5B4.1, Cat. No. MABE644, is a highly specific mouse monoclonal antibody that targets Cre recombinase and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant Cre lacking the N-terminal region sequence.
      Clone5B4.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5B4.1 detects an epitope outside the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteVirus
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • cre
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight38.54 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in recombinant Cre.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected 250 ng of recombinant Cre (with added N- and C-terminal tag sequences).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE644 04054839086113