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NA61 Anti-BrdU (Ab-3) Mouse mAb (Mobu-1)

Anti-BrdU (Ab-3), mouse monoclonal, clone Mobu-1, recognizes BrdU. Requires biosynthetic labeling of the target cells with BrdU. It is validated for ICC and use with frozen and paraffin sections.

Anti-BrdU (Ab-3) Mouse mAb (Mobu-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bromodeoxyuridine

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NA61
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

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NA61-100UG
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      Description
      OverviewRecognizes BrdU (bromodeoxyuridine). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine. This is the preferred BrdU antibody for staining.
      Catalogue NumberNA61
      Brand Family Calbiochem®
      SynonymsAnti-Bromodeoxyuridine
      Application Data
      Detection of BrdU-labeled DNA by immunocytochemistry. Sample: A431 cells. Primary antibody: Anti-BrdU (Ab-3) Mouse mAb (Mobu-1) (Cat. No. NA61) (1 µg/ml). Detection: fluorescence.
      References
      ReferencesGratzner, H.G. 1982. Science 218, 474.
      Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385.
      Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative controlUnlabeled cells
      Positive controlBrdU labeled DNA
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Schutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343.
      Key Applications Frozen Sections
      Immunocytochemistry
      Not Western Blot
      Paraffin Sections
      Application NotesFrozen Sections (2 µg/ml)
      Immunocytochemistry (1 µg/ml)
      Paraffin Sections (2.5 µg/ml, trysin pre-treatment required)
      Immunoblotting (not recommended)
      Application CommentsTo enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized, and the DNA denatured. This is done in one step by treatment with Fixative/Denaturing Solution (70% EtOH, 0.1 N NaOH). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine (Cat. No. 203806). Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 minutes. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. Antibody should be titrated for optimal results in individual system.
      Biological Information
      ImmunogenBrdU-labeled DNA
      CloneMobu-1
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NA61-100UG 04055977227604

      Documentation

      Anti-BrdU (Ab-3) Mouse mAb (Mobu-1) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-BrdU (Ab-3) Mouse mAb (Mobu-1) Certificates of Analysis

      TitleLot Number
      NA61

      References

      Reference overview
      Gratzner, H.G. 1982. Science 218, 474.
      Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385.
      Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      SynonymsAnti-Bromodeoxyuridine
      ApplicationFrozen Sections (2 µg/ml)
      Immunocytochemistry (1 µg/ml)
      Paraffin Sections (2.5 µg/ml, trysin pre-treatment required)
      Immunoblotting (not recommended)
      Application Data
      Detection of BrdU-labeled DNA by immunocytochemistry. Sample: A431 cells. Primary antibody: Anti-BrdU (Ab-3) Mouse mAb (Mobu-1) (Cat. No. NA61) (1 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-653 mouse myeloma cells (see application references). Recognizes BrdU (bromodeoxyuridine).
      BackgroundProliferating cells are usually detected by incorporation of [3H]-thymidine into DNA and quantitation by autoradiography or scintillation counting. Alternatively, bromodeoxyuridine (BrdU), an analog of thymidine, can be incorporated specifically into the DNA of replicating cells and the extent of incorporation can be detected using antibodies directed against the incorporated analog. The proportion of cells actively proliferating can then be determined by immunofluorescence or flow cytometry as well as immunohistochemistry (which has been done with tumors, tissues, and blood cell elements).
      HostMouse
      ImmunogenBrdU-labeled DNA
      CloneMobu-1
      IsotypeIgG₁
      Speciesa broad range of species
      Positive controlBrdU labeled DNA
      Negative controlUnlabeled cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsTo enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized, and the DNA denatured. This is done in one step by treatment with Fixative/Denaturing Solution (70% EtOH, 0.1 N NaOH). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine (Cat. No. 203806). Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 minutes. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. Antibody should be titrated for optimal results in individual system.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesGratzner, H.G. 1982. Science 218, 474.
      Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385.
      Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.
      Application referencesOriginal Clone Schutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343.