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AM04 Anti-Bak (Ab-2) Mouse mAb (TC-102)

Anti-Bak (Ab-2), mouse monoclonal, clone TC-102, recognizes the ~30 kDa Bak protein in A431, HeLa, MCF-7, and SKBR3 cells. It is validated for Western blotting and immunofluorescence.

Anti-Bak (Ab-2) Mouse mAb (TC-102) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-2 Antagonist Killer

AM04
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~30 kDa (apparent MW) Bak protein in A431, HeLa, MCF-7 or SKBR3 cells.
      Catalogue NumberAM04
      Brand Family Calbiochem®
      SynonymsAnti-Bcl-2 Antagonist Killer
      References
      ReferencesChittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative controlSKBR3 cells
      Positive controlA431 cells, HeLa cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (5 µg/ml)
      Immunofluorescence (1 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems for each application.
      Biological Information
      Immunogenhuman recombinant Bak protein from which the C-terminal and transmembrane domains have been deleted
      ImmunogenHuman
      Epitopewithin the N-terminal region
      CloneTC-102
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM04 0

      Documentation

      Anti-Bak (Ab-2) Mouse mAb (TC-102) MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Bak (Ab-2) Mouse mAb (TC-102) Certificates of Analysis

      TitleLot Number
      AM04

      References

      Reference overview
      Chittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-July-2010 JSW
      SynonymsAnti-Bcl-2 Antagonist Killer
      ApplicationImmunoblotting (5 µg/ml)
      Immunofluorescence (1 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~30 kDa (apparent MW) Bak protein.
      BackgroundBak (for bcl-2 homologous antagonist/killer) is a member of the bcl-2 family of proteins. It was independently identified by three different groups using either the two hybrid system for identifying adenovirus E1B 19K interacting proteins or by PCR cloning using degenerate BH1 and BH2 domain primers derived from the human bcl-2 gene. Bak shows a 53% homology with bcl-2 in the BH1 and BH2 domains but an overall homology of 28% with bcl-2. The presence of a hydrophobic tail on the protein suggests that, like bcl-2, it is membrane localized. The bak protein is translated from a 2.2 kb mRNA to yield a 211-216 amino acid protein with a predicted molecular weight of 23.4 kDa, but which migrates by gel electrophoresis as an approximately 30 kDa protein under denaturing conditions. Bak is expressed widely in many tissues including heart, thymus, and colon. The bak gene (bak-1) has been mapped to chromosome 6 and spans approximately 6 kb of DNA3. Southern analysis has indicated the presence of two additional bak genes: bak-2 has 97% homology with bak-1 and maps to chromosome 20, and bak-3, a pseudogene, which maps to chromosome 11. Functionally, bak is similar to bax in that it induces apoptosis. The protein pairs with bcl-xL but unlike its pairing partner has a much more extensive tissue distribution, being present in most tissues tested. While conflicting reports exist for the heterodimerization of bak with other members of the bcl-2 family of proteins, it is clear that bak does partner with the E1B 19K protein.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman recombinant Bak protein from which the C-terminal and transmembrane domains have been deleted
      Epitopewithin the N-terminal region
      CloneTC-102
      IsotypeIgG2b
      Specieshuman, mouse
      Positive controlA431 cells, HeLa cells
      Negative controlSKBR3 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems for each application.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesChittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.