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OP95 Anti-BRCA2 (Ab-1) Mouse mAb (2B)

Anti-BRCA2 (Ab-1), mouse monoclonal, clone 2B, recognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. It is validated for Western blotting and immunoprecipitation.

Anti-BRCA2 (Ab-1) Mouse mAb (2B) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Breast Cancer Susceptibility Protein

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OP95
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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OP95-100UG
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      Description
      OverviewRecognzies the ~460 kDa BRCA2 protein in cells expressing a wild type BRCA2 gene. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
      Catalogue NumberOP95
      Brand Family Calbiochem®
      SynonymsAnti-Breast Cancer Susceptibility Protein
      References
      ReferencesAndres, J.L., et al. 1998. Oncogene 16, 2229.
      Chen, J., et al. 1998. Mol. Cell 2, 317.
      Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 5287.
      Marmorstein, L.Y., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13869.
      Connor, F., et al. 1997. Nat. Genet. 17, 423.
      Sharan, S.K., et al. 1997. Nature 386, 804.
      Ludwig, T., et al. 1997. Genes Dev. 11, 1226.
      Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
      Rajan, J.V., et al. 1997. Dev. Biol. 184, 385.
      Suzuki, A., et al. 1997. Genes Dev. 11, 1242.
      Wong, A.K.C., et al. 1997. J. Biol. Chem. 272, 31941.
      Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin. Oncol. 23, 1.
      Goggins, M., et al. 1996. Cancer Res. 56, 5360.
      Stratton, M.R. 1996. Hum. Mol. Genet. 5, 1515.
      Tavtigian, S.V., et al. 1996. Nat. Genet. 12, 333.
      Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13078.
      Spillman, M.A. and A.M. Bowcock. 1996. Oncogene 13, 1639.
      Vaughn, J.P., et al. 1996. Cancer Res. 56, 4590.
      Wooster, R., et al. 1995. Nature 378, 789.
      Product Information
      DeclarationSold under license of U.S. Patents 5,837,492 and 6,124,104.
      FormLiquid
      FormulationIn PBS.
      Positive controlMCF7 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (2 µg/ml)
      Immunoprecipitation (2 µg)
      Application CommentsThe CAPAN-1 cell line, which has 6174delT mutation in the single BRCA2 allele, expresses a truncated BRCA2 protein of 230 kDa that contains the Anti-BRCA2 epitope and retains RAD51 binding but has lost p53 binding. Full length BRCA2 protein will co-migrate on SDS-PAGE with the catalytic subunit of DNA PK which can be detected using Anti-DNA PK, Cat. No. PC127. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1651-1821 of human BRCA2 fused to GST
      ImmunogenHuman
      Clone2B
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      OP95-100UG 04055977209709