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MAB4145 Anti-BCRP Antibody, clone BXP-34

Detect BCRP using this Anti-BCRP Antibody, clone BXP-34 validated for use in IC, IH.

Anti-BCRP Antibody, clone BXP-34 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Hematopoietic Stem Cells Gene Symbol: ABCG2, BCRP, BMDP, BCRP1, MXR, ABC15, ABCP, EST157481, MGC102821, MXR1, MRX, CDw338 UniProt Number: Q9UNQ0
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MAB4145
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHCMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMAB4145
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BCRP Antibody, clone BXP-34
      Alternate Names
      • Breast Cancer Resistance Protein
      • Placenta-specific ATP Binding Cassette Transporter
      • ATP-binding Cassette, Sub Family G, member 2 (ABCG2)
      Background InformationThe 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • MCF-7 cells
      PresentationLiquid containing 0.7% BSA and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect BCRP using this Anti-BCRP Antibody, clone BXP-34 validated for use in IC, IH.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Applications Not Recommended
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations

      Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections

      Not suitable for paraffin-embedded tissues or for Western blotting

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenThe Mitoxanthrone resistant, BCRP-overexpressing cell line MCF7-MR.
      CloneBXP-34
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
      Gene Symbol
      • ABCG2
      • BCRP
      • BMDP
      • BCRP1
      • MXR
      • ABC15
      • ABCP
      • EST157481
      • MGC102821
      • MXR1
      • MRX
      • CDw338
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UNQ0 # Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123.
      SIZE: 655 amino acids; 72314 Da
      SUBUNIT: Monomer or homodimer; disulfide-linked.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Highly expressed in placenta. Low expression in small intestine, liver and colon.
      PTM: Glycosylation-deficient ABCG2 is normally expressed and functional.
      SIMILARITY: SwissProt: Q9UNQ0 ## Belongs to the ABC transporter family. ABCG (White) subfamily. & Contains 1 ABC transporter domain.
      Stem Cell Type
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      Molecular Weight70 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4145 04053252722981

      Documentation

      Anti-BCRP Antibody, clone BXP-34 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-BCRP Antibody, clone BXP-34 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-BCRP MONOCLONAL ANTIBODY - 2137009 2137009
      MOUSE ANTI-BCRP - 2537870 2537870
      MOUSE ANTI-BCRP - 3051542 3051542
      MOUSE ANTI-BCRP - 3829524 3829524
      MOUSE ANTI-BCRP - 4006702 4006702
      MOUSE ANTI-BCRP - 4069766 4069766
      MOUSE ANTI-BCRP - 4187700 4187700
      MOUSE ANTI-BCRP - 4213776 4213776
      MOUSE ANTI-BCRP -2544322 2544322
      MOUSE ANTI-BCRP -2547211 2547211
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies