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MABE144 Anti-Ago3 Antibody, clone 4B1-F6

Anti-Ago3 Antibody, clone 4B1-F6 is a Mouse Monoclonal Antibody for detection of Ago3 also known as eIF-2C 3, eIF2C 3, FLJ12765, MGC86946 & has been validated in ICC & IP.

Anti-Ago3 Antibody, clone 4B1-F6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: EIF2C3, AGO3 UniProt Number: Q9H9G7
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MABE144
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE144
      DescriptionAnti-Ago3 Antibody, clone 4B1-F6
      Alternate Names
      • eIF-2C 3
      • eIF2C 3
      • FLJ12765
      • MGC86946
      Background InformationEukaryotic translation initiation factor 2C 3, Ago3, belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain.
      Ago3 is indicated to play a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. Unlike Ago1 and Ago2, Ago3 and AG04 do not possess the ability to dissociate miRNA duplexes in a minimal in vitro system. Re-introduction of any Ago variant into embyronic (ES) cells deficient for expression of all four Ago variants will rescue miRNA silencing defects and reduce apoptosis.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cells
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ago3 Antibody, clone 4B1-F6 is a Mouse Monoclonal Antibody for detection of Ago3 also known as eIF-2C 3, eIF2C 3, FLJ12765, MGC86946 & has been validated in ICC & IP.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Azuma-Mukai, A., et al. (2008). Proc Natl Acad Sci. USA. 105(23):7964-7969.)
      Biological Information
      ImmunogenRecombinant protein corresponding to human Ago3.
      Clone4B1-F6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      Specificity This antibody has been shown to not cross react with Ago1, Ago2, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene.
      Gene Symbol
      • EIF2C3
      • AGO3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.

      SUBUNIT STRUCTURE: Interacts with EIF4B, IMP8, PRMT5 and TNRC6B.

      SUBCELLULAR LOCATION: Cytoplasm > P-body.
      Molecular Weight96 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Ago3 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE144 04053252754395

      Documentation

      Anti-Ago3 Antibody, clone 4B1-F6 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Ago3 Antibody, clone 4B1-F6 Certificates of Analysis

      TitleLot Number
      Anti-Ago3, clone 4B1-F6 - 2391171 2391171
      Anti-Ago3, clone 4B1-F6 - 2006946 2006946
      Anti-Ago3, clone 4B1-F6 - 3310131 3310131
      Anti-Ago3, clone 4B1-F6 - 3433738 3433738
      Anti-Ago3, clone 4B1-F6 - 3808724 3808724
      Anti-Ago3, clone 4B1-F6 - NRG1911092 NRG1911092
      Anti-Ago3, clone 4B1-F6 -2524850 2524850
      Anti-Ago3, clone 4B1-F6 -2775321 2775321
      Anti-Ago3, clone 4B1-F6 -2786916 2786916
      Anti-Ago3, clone 4B1-F6 Monoclonal Antibody 2929151

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies