Millipore Sigma Vibrant Logo

287810 2ʹ,7ʹ-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.

2ʹ,7ʹ-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: H₂DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate

View Products on Sigmaaldrich.com
287810
View Pricing & Availability

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
4091-99-0C₂₄H₁₆Cl₂O₇

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
287810-100MG
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 100 mg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      OverviewCell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.
      Catalogue Number287810
      Brand Family Calbiochem®
      SynonymsH₂DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate
      References
      ReferencesCrow, J.P. 1997. Nitric Oxide 1, 145.
      Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
      Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta 1291, 245.
      Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
      Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
      Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
      Product Information
      CAS number4091-99-0
      ATP CompetitiveN
      FormCrystalline solid
      Hill FormulaC₂₄H₁₆Cl₂O₇
      Chemical formulaC₂₄H₁₆Cl₂O₇
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetDetection of reactive oxygen species (ROS) and nitric oxide (•NO)
      Purity≥94% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      287810-100MG 07790788054298

      Documentation

      2ʹ,7ʹ-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      2ʹ,7ʹ-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem Certificates of Analysis

      TitleLot Number
      287810

      References

      Reference overview
      Crow, J.P. 1997. Nitric Oxide 1, 145.
      Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
      Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta 1291, 245.
      Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
      Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
      Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-April-2010 RFH
      SynonymsH₂DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate
      DescriptionCell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and the nitric oxide (NO) in neutrophils and macrophages and for the determination of the overall oxidative stress in toxicological phenomena.
      FormCrystalline solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number4091-99-0
      Chemical formulaC₂₄H₁₆Cl₂O₇
      Structure formulaStructure formula
      Purity≥94% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesCrow, J.P. 1997. Nitric Oxide 1, 145.
      Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
      Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta 1291, 245.
      Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
      Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
      Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.