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SCC443 12Z Human Endometrial Epithelial Cell Line

The N-cadherin-positive 12Z human endometrial cell line is an established model for tissue invasion in endometriosis, and is used to study disease progression, pathogenesis, and therapeutic intervention.

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SCC443
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC443
      Description12Z Human Endometrial Epithelial Cell Line
      Alternate Names
      • endometrial cell line, endometriosis cell line, 12Z
      Background InformationEndometriosis is a gynecologic condition that is characterized by the presence of endometrial tissue outside of the uterus. This condition affects approximately 6-10% of all women and can lead to significant pelvic pain and infertility. The limited availability of in vitro models hinders cellular, molecular, and pathophysiologic studies of endometriosis which translates to poor understanding of the disease and limited available treatments.
      Several endometriotic cell lines were developed by researchers from Goethe University Frankfurt in Germany. One of these is the 12Z cell line which was derived from the endometrial peritoneum of a 37 year-old female undergoing laparoscopy. The 12Z cell line was immortalized using SV40 plasmid transfection. This cell line expresses both pan-cytokeratin and N-Cadherin.
      One of the frequently-studied means for the initiation of endometriosis is retrograde menstruation that permits the deposition of endometrial tissues outside of the uterus. During this process, the invasive capabiity of cells is believed to be essential.
      The 12Z immortalized human endometrial epithelial cell line is an established model for endometriosis as it is derived from endometriotic tissue that is positive for N-Cadherin+, a marker for invasiveness. This makes the 12Z iine a suitable model for the study of disease progression or therapeutic development. The 12Z cell line is frequently used in 2D or 3D invasion assays, as well as in biochemical assays to study the pathophysiology of endometriosis.

      Source
      The 12Z cell line was derived from endometrial peritoneum biopsy of a 37 year-old female. This cell line was immortalized via transfection of a plasmid containing SV40T.

      References
      1. Nat. Rev. Endocrinol 2019, 15(11): 666-682.
      2. Lancet 2004, 364 (9447): 1789-1799
      3. Am. J. Pathol 2001, 159(5): 1839-1852
      4. Reproductive BioMedicine Online 2022, 44(6): 976-990. DOI: https://doi.org/10.1016/j.rbmo.2022.02.008
      5. Sci Rep 2021, 11(1): 4115
      References
      Product Information
      HS Code3002 59 00
      Quality LevelMQ100
      Applications
      ApplicationThe N-cadherin-positive 12Z human endometrial cell line is an established model for tissue invasion in endometriosis, and is used to study disease progression, pathogenesis, and therapeutic intervention.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Epithelial Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach vial contains ≥1X10⁶ viable cells.

      Cells are tested negative for infectious diseases by a Human Essential CLEAR Panel by Charles River Animal Diagnostic Services.

      Cells are verified to be of human origin and negative for interspecies contamination from rat, mouse, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services

      Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsStore cells in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and function.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC443 04065268850961