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70934 pTriEx™-4 Hygro DNA

pTriEx™-4 Hygro DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

CoA
User Protocol
Citations
Vector Map
Vector Sequence

Recommended Products

Overview

Replacement Information

Description
Overview	This product has been discontinued. The pTriEx™-4 Hygro vector is designed to allow rapid characterization of target genes in multiple expression systems, and to allow rapid selection of stable transfected mammalian cells expressing high levels of target gene. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Expression in vertebrate cells is mediated by the CMV immediate early enhancer and promoter². The drug selection marker is expressed under the control of the EMC virus Cap-Independent Translation Enhancer (CITE) sequence (or IRES) allowing rapid selection of transfected mammalian cells using hygromycin. For expression in insect cells, pTriEx-4 Hygro contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector^® System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λlp_L and λp_I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows induction with IPTG. Native protein can be expressed by cloning into the Nco I site, or generated by cloning into the PshA I or Sma I sites and cleaving the fusion protein with enterokinase or thrombin, respectively. The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number	70934
Brand Family	Novagen®

Description

Overview

This product has been discontinued.

The pTriEx™-4 Hygro vector is designed to allow rapid characterization of target genes in multiple expression systems, and to allow rapid selection of stable transfected mammalian cells expressing high levels of target gene. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Expression in vertebrate cells is mediated by the CMV immediate early enhancer and promoter². The drug selection marker is expressed under the control of the EMC virus Cap-Independent Translation Enhancer (CITE) sequence (or IRES) allowing rapid selection of transfected mammalian cells using hygromycin. For expression in insect cells, pTriEx-4 Hygro contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector^® System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λlp_L and λp_I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows induction with IPTG. Native protein can be expressed by cloning into the Nco I site, or generated by cloning into the PshA I or Sma I sites and cleaving the fusion protein with enterokinase or thrombin, respectively.

The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Catalogue Number

70934

Brand Family

Novagen®

References

Product Information

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped with Blue Ice or with Dry Ice
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
70934	0

Documentation

pTriEx™-4 Hygro DNA Certificates of Analysis

Title	Lot Number
70934	Enter Lot Number

Citations

Title

Mahito Sadaie, Kaori Shinmyozu and Jun-ichi Nakayama. (2008) A conserved SET-domain methyltransferase, Set11, modifies ribosomal protein Rpl12 in fission yeast. Journal of Biological Chemistry 283, 7185-7195.

Xue-Ming Xu, et al. (2005) Evidence for direct roles of two additional factors, SECp43 and soluble liver antigen, in the selenoprotein synthesis machinery. Journal of Biological Chemistry 280, 41568-41575.

User Protocols

Title
TB053 Academic and Non-profit Laboratory Assurance Letter
TB250 pTriEx™ System Manual

Vector Map

Title
TB300 pTriEx-4 Hygro Vector Map

Vector Sequence

Title
pTriEx-4 Hygro Sequence

Related Products & Applications

Related Products

Catalogue Number	Description
70927	pTriEx™-1.1 Neo DNA - Novagen
70928	pTriEx™-1.1 Hygro DNA - Novagen
70929	pTriEx™-2 Neo DNA - Novagen
70930	pTriEx™-2 Hygro DNA
70931	pTriEx™-3 Neo DNA - Novagen
70932	pTriEx™-3 Hygro DNA
70933	pTriEx™-4 Neo DNA - Novagen

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70934 pTriEx™-4 Hygro DNA

Recommended Products

Overview

Documentation

pTriEx™-4 Hygro DNA Certificates of Analysis

Citations

User Protocols

Vector Map

Vector Sequence

Related Products & Applications

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