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70826 Sigma-AldrichpTriEx™-2 DNA

pTriEx™-2 DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Brochures
User Protocol
Citations
Vector Map
Vector Sequence

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Overview

Replacement Information

Description
Overview	This product has been discontinued. The pTriEx™-2 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by a hybrid promoter composed of the CMV immediate early enhancer fused to the chicken β-actin promoter. For expression in insect cells, pTriEx-2 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λp_L and λlp_I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. Native protein can be expressed by cloning into the NcoI site. Alternatively, native protein can be generated by cloning into the PshA I or Sma I sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively. The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number	70826
Brand Family	Novagen®

Description

Overview

This product has been discontinued.

The pTriEx™-2 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector a single recombinant plasmid can be used to test expression in E. coli, insect and vertebrate cells. Transient vertebrate expression is mediated by a hybrid promoter composed of the CMV immediate early enhancer fused to the chicken β-actin promoter. For expression in insect cells, pTriEx-2 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λp_L and λlp_I promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. Native protein can be expressed by cloning into the NcoI site. Alternatively, native protein can be generated by cloning into the PshA I or Sma I sites and cleavage of the fusion protein with Enterokinase or Thrombin, respectively.

The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Catalogue Number

70826

Brand Family

Novagen®

References

Product Information
Declaration	This product is not available in Japan.

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped with Blue Ice or with Dry Ice
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
70826	0

Documentation

pTriEx™-2 DNA SDS

Title
Safety Data Sheet (SDS)

pTriEx™-2 DNA Certificates of Analysis

Title	Lot Number
70826	Enter Lot Number

Brochure

Title
Insect Cell Expression

Citations

Title

Trine Nilsen, et al. (2007) A nuclear export sequence located on a β-strand in fibroblast growth factor-1. Journal of Biological Chemistry 282, 26245-26256.

Katherine J. Strissel, et al. (2005) Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors. Journal of Biological Chemistry 280, 29250-29255.

Jedrzej Malecki, et al. (2004) Translocation of FGF-1 and FGF-2 across vesicular membranes occurs during G1-phase by a common mechanism. Molecular Biology of the Cell 15, 801-814.

User Protocols

Title
TB053 Academic and Non-profit Laboratory Assurance Letter
TB250 pTriEx™ System Manual

Vector Map

Title
TB277 pTriEx™-2 Vector Map

Vector Sequence

Title
pTriEx-2 Sequence

Catalogue Number	Description
70823	pTriEx™-3 DNA - Novagen
70824	pTriEx™-4 DNA - Novagen
70840	pTriEx™-1.1 DNA - Novagen

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70826 Sigma-AldrichpTriEx™-2 DNA

Recommended Products

Overview

Documentation

pTriEx™-2 DNA SDS

pTriEx™-2 DNA Certificates of Analysis

Brochure

Citations

User Protocols

Vector Map

Vector Sequence

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