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71731 pIEx/Bac™-3 3C/LIC Vector Kit - Novagen

Novagen's pIEx/Bac vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells.

pIEx/Bac™-3 3C/LIC Vector Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71731
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



The pIEx/Bac™-3 3C/LIC vector is a 3C/LIC prepared version of pIEx/Bac-3. The pIEx/Bac™ vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10 histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence.
Catalogue Number71731
Brand Family Novagen®
References
Product Information
•20 µg (40 µl) 0.5 µg/µl pIEx/Bac-3 3C/LIC DNA
•50 µlT4 DNA Polymerase Buffer
•5 µg (10 µl) 0.5 µg/µl pIEx/Bac 3C/LIC Positive Expression Control
•25 UT4 DNA Polymerase, LIC-qualified
•100 µl100 mM DTT
•40 µl25 mM dATP
•50 µl25 mM EDTA
•1.5 ml Nuclease-free Water
•1 pkgNovaBlue GigaSingles Competent Cells
•2 mlSOC Medium
•10 µl Test Plasmid
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71731 0