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70779 pET Expression System 29

pET vectors plus host strains with induction control

pET Expression System 29 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70779
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 29
The pET Expression System 29 contains 10 µg each of the four versions of pET-29 (pET-29a–c(+)). The pET-29a–c(+) vectors carry an N-terminal S•Tag™/thrombin configuration plus an optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB076VM.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number70779
Brand Family Novagen®
References
Product Information
•10 µg eachpET-29a-c(+) vector DNA
•0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
•0.2 mlInduction control clone, glycerol stock
Fusion tagHis•Tag, S•Tag
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70779 0

Documentation

pET Expression System 29 Certificates of Analysis

TitleLot Number
70779

Citations

Title
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  • Michael A. Fischbach, et al. (2007) Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes. Procedings of the National Academy of Science 104, 11951-11956.
  • Caterina Grillo, et al. (2007) DNA-binding activity of the ERp57 C-terminal domain is related to a redox-dependent conformational change. Journal of Biological Chemistry 282, 10299-10310.
  • Tomasz Kulikowicz and Theresa A. Shapiro. (2006) Distinct genes encode type II topoisomerases for the nucleus and mitochondrion in the protozoan parasite Trypanosoma brucei. Journal of Biological Chemistry 281, 3048-3056.
  • Peter T. Beernink, et al. (2005) Specificity of protein interactions mediated by BRCT domains of the XRCC1 DNA repair protein. Journal of Biological Chemistry 280, 30206-30213.
  • Maria- Eugenia Guazzaroni, et al. (2005) The multidrug efflux regulator TtgV recognizes a wide range of structurally different effectors in solution and complexed with target DNA. Evidence from isothermal titration calorimetry. Journal of Biological Chemistry 280, 20887-20893.
  • Ia-Wen Hsu, et al. (2005) Phosphorylation of Y14 modulates its interaction with proteins involved mRNA metabolism and influences its methylation. Journal of Biological Chemistry 280, 34507-34512.
  • Lucia B. Jilaveanu, Christopher R. Zito and Donald Oliver. (2005) Dimeric SecA is essential for protein translocation. Proceedings of the National Academy of Sciences (USA) 102, 7511-7516.
  • Alla Korepanova, et al. (2005) Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli. Protein Science 14, 148-158.
  • Maria V. Lara, et al. (2005) NADP-malic enzyme and Hsp70: co-purification of both proteins and modification of NADP-malic enzyme properties by association with Hsp70. Plant and Cell Physiology 46, 997-1006.
  • Pei-Hsun Lin and Sue Lin-Chao. (2005) RhlB helicase rather than enolase is the {beta}-subunit of the Escherichia coli polynucleotide phosphorylase (PNPase)-exoribonucleolytic complex. Proceedings of the National Academy of Sciences (USA) 102, 16590-16595.
  • Andrew L. Lovering, et al. (2005) Mechanistic and structural analysis of a family 31 -glycosidase and Its glycosyl-enzyme intermediate. Journal of Biological Chemistry 280, 2105-2115.
  • Johannes Mullegger, et al. (2005) Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts. Protein Engineering Design and Selection 18, 33-40.
  • Takashi Nagaike, et al. (2005) Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase. Journal of Biological Chemistry 280, 19721-19727.
  • Ju-Ock Nam, et al. (2005) Regulation of tumor angiogenesis by fastatin, the fourth FAS1 domain of β-ig-h3, via α-v-β-3 integrin. Cancer Research 65, 4153-4161.
  • Seung Bae Rho, et al. (2005) The identification of apoptosis-related residues in human thymosin -10 by mutational analysis and computational modeling. Journal of Biological Chemistry 280, 34003-34007.
  • S. Dean Rider, et al. (2005) The protozoan parasite Cryptosporidium parvum possesses two functionally and evolutionarily divergent replication protein A large subunits. Journal of Biological Chemistry 280, 31460-31469.
  • Wei Zhang, John S. Olson and George N. Phillips, Jr.. (2005) Biophysical and kinetic characterization of HemAT, an aerotaxis receptor from Bacillus subtilis. Biophysical Journal 88, 2801-2814.
  • Christopher R. Zito, et al. (2005) Role of a conserved glutamate residue in the E. coli SECA ATPASE mechanism. Journal of Biological Chemistry 280, 14366-14369.
  • David M. Rancour, et al. (2004) Plant UBX domain-containing protein 1, PUX1, regulates the oligomeric structure and activity of Arabidopsis CDC48. Journal of Biological Chemistry 279, 54264-54274.
  • Kunhong Xiao, et al. (2004) Activation-dependent conformational changes in β-arrestin 2. Journal of Biological Chemistry 279, 55744-55753.
  • Timur I. Gaynutdinov, et al. (2003) Chimeric ribonuclease as a source of human adapter protein for targeted drug delivery. 16, 771-775.
  • Tatiana Suarez, Subhasis B. Biswas and Esther E. Biswas. (2002) Biochemical defects in retina-specific human ATP binding cassette transporter nucleotide binding domain 1 mutants associated with macular degeneration. Journal of Biological Chemistry 277, 21759-21767.
  • Valerie Chazalet, et al. (2001) Identification of essential amino acids in the Azorhizobium caulinodans fucosyltransferase nodZ. Journal of Bacteriology 183, 7067-7075.
  • Yulin Jia, et al. (2000) Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. European Molecular Biology Organization Journal 19, 4004-4014.
  • Eiko Kanaya, et al. (1999) Characterization of the transcriptional activator CBF1 from Arabidopsis thaliana. Journal of Biological Chemistry 274, 16068-16076.
  • I-Cheng Ho, et al. (1996) The proto-oncogene c-maf is responsible for tissue-specific expression of interleukin-4. Cell 85, 973-983.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB076VM pET-29a-c(+) Vector Map

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