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70759 pET Expression System 19b - Novagen

pET vectors plus host strains with induction control

pET Expression System 19b - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70759
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock

Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid

These components are sufficient for up to 10 transformations in each host.

Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

pET Expression System 19b
The pET-19b vector carries an N-terminal His•Tag® sequence followed by an enterokinase cleavage site and three cloning sites. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB049VM.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number70759
Brand Family Novagen®
References
Product Information
Components
Fusion tagHis•Tag
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70759 0

Documentation

pET Expression System 19b - Novagen Certificates of Analysis

TitleLot Number
70759

Citations

Title
  • Barbara Becattini, et al. (2006) Structure-activity relationships by interligand NOE-based design and synthesis of antiapoptotic compounds targeting Bid. Procedings of the National Academy of Science 103, 12602-12606.
  • Andrew D. Ferguson, et al. (2006) NMR structures of the selenoproteins sep15 and selM reveal redox activity of a new thioredoxin-like family. Journal of Biological Chemistry 281, 3536-3543.
  • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
  • Micah M. Bhatti and William J. Sullivan, Jr.. (2005) Histone acetylase GCN5 enters the nucleus via importin- in protozoan parasite Toxoplasma gondii. Journal of Biological Chemistry 280, 5902-5908.
  • Marcelo Comini, et al. (2005) Trypanothione synthesis in Crithidia revisited. Journal of Biological Chemistry 280, 6850-6860.
  • Eric S. Folker, Brian M. Baker and Holly V. Goodson. (2005) Interactions between CLIP-170, tubulin, and microtubules: implications for the mechanism of CLIP-170 plus-end tracking behavior. Molecular Biology of the Cell 16, 5373-5384.
  • Antony M. Jose and Michael R. Koelle. (2005) Domains, amino acid residues, and new isoforms of Caenorhabditis elegans diacylglycerol kinase 1 (DGK-1) important for terminating diacylglycerol signaling in vivo. Journal of Biological Chemistry 280, 2730-2736.
  • Vyacheslav M. Labunskyy, et al. (2005) A novel cysteine-rich domain of SEP15 mediates the interactions with UDP-glucose: Glycoprotein glucosyltransferase. Journal of Biological Chemistry 280, 37839-37845.
  • Rory E. Morty, et al. (2005) Oligopeptidase B from Trypanosoma evansi: a parasite peptidase that inactivates atrial natriuretic factor in the bloodstream of infected hosts. Journal of Biological Chemistry 280, 10925-10937.
  • Belinda J. Hales, et al. (2004) Isoforms of the major peanut allergen arah2: IgE binding in children with peanut allergy. International Archives of Allergy and Immunology 135, 101-107.
  • Yuhong Wang, et al. (2004) Human myoglobin recognition of oxygen: dynamics of the energy landscape. Procedings of the National Academy of Science 101, 18000-18005.
  • Michael Albers, et al. (2003) Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors. RNA 9, 138-150.
  • Edward J. Kim and David M. Helfman. (2003) Characterization of the metastasis-associated protein, S100A4. Journal of Biological Chemistry 278, 30063-30073.
  • G. Chang, et al. (1998) Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science 282, 2220-2226.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB049VM pET-19b Vector Map

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