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23-024M p27 Protein complex, activated, 250 µg

Activated complex of three full length, recombinant human proteins: p27, Cyclin E1 & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays.

p27 Protein complex, activated, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
23-024M
250 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesCascade Component
Enzyme AssaysHuman Substrate
Description
Catalogue Number23-024M
Brand Family Upstate
Trade Name
  • Upstate
Descriptionp27 Protein complex, activated, 250 µg
OverviewAXIS Axon Isolation Devices, 500 µm long channels, 10 devices
Alternate Names
  • p27kip1
  • Cyclin-dependent kinase inhibitor 1B
  • Kip1
Background Informationp27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActivated complex of three full length, recombinant human proteins: p27, Cyclin E1 & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays.
Key Applications
  • Enzyme Assays
Biological Information
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade ComponentSubstrate
Entrez Gene Number
Purification MethodPurified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size250 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
23-024M 04053252294402

Related Products & Applications

Alternative Packsize

Catalogue Number Description
23-024 p27 Protein complex, activated, 10 µg

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Categories

Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitination Substrates
Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Ubiquitination Substrates