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500554 mTOR Activator, MHY1485 - CAS 326914-06-1 - Calbiochem

mTOR Activator, MHY1485, CAS Number: 326914-06-1, is a cell-permeable activator of mTOR that inhibits autophagy by suppressing the fusion between autophagosomes and lysosomes.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III

500554
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
326914-06-1C₁₇H₂₁N₇O₄

Products

Catalogue NumberPackaging Qty/Pack
5.00554.0001 Glass bottle 10 mg
Description
OverviewA cell-permeable triazine derivative that is shown to increase cellular mTOR Ser2448 and downstream substrate 4E-BP Thr37/46 phosphorylation level in rat liver Ac2F cells (1 to 2 µM for 1 h), affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes. The mechanism of mTOR activation by MHY1485 is currently unknown.
Catalogue Number500554
Brand Family Calbiochem®
Synonyms4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III
References
ReferencesChoi, Y. J., et al. 2012. PLoS. One. 7, e43418.
Product Information
CAS number326914-06-1
FormPale yellow solid
Hill FormulaC₁₇H₂₁N₇O₄
Chemical formulaC₁₇H₂₁N₇O₄
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationmTOR Activator, MHY1485, CAS Number: 326914-06-1, is a cell-permeable activator of mTOR that inhibits autophagy by suppressing the fusion between autophagosomes and lysosomes.
Biological Information
Primary Target IC<sub>50</sub>~2 µ
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
5.00554.0001 04055977244922

Documentation

mTOR Activator, MHY1485 - CAS 326914-06-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Choi, Y. J., et al. 2012. PLoS. One. 7, e43418.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-May-2013 JSW
Synonyms4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III
DescriptionA cell-permeable triazine derivative that is shown to increase cellular phosphorylation level of mTOR Ser2448 (20% and 42% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) and downstream substrate 4E-BP Thr37/46 (29% and 34% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) in rat liver Ac2F cells, affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F (117%, 150%, and 250% of vehicle control LC3-II/LC3-I ratio, respectively, with 0.5, 1, and 2 µM treatment for 6 h; 142% and 172% of control ratio, respectively, with 2 µM treatment for 1 h or 12 h) is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes as demonstrated by a reduction of starvation-induced AdGFP-LC3 and lysosome dye co-localization (27% and 32.7% above control co-localization in 6 h serum deprived cultures with or without 2 µM MHY1485; 21% above control co-localization with 2 µM MHY1485 treatment alone and without serum starvation). The mechanism of mTOR activation by MHY1485 is currently unknown.
FormPale yellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number326914-06-1
Chemical formulaC₁₇H₂₁N₇O₄
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (12.5 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesChoi, Y. J., et al. 2012. PLoS. One. 7, e43418.