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QIA10 c-ErbB2/c-Neu Rapid Format ELISA Kit

c-ErbB2/c-Neu Rapid Format ELISA Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: c-Neu ELISA Kit, ErbB2 ELISA Kit, Erythroblastosis Virus ELISA Kit, HER-2 ELISA Kit, HER2 ELISA Kit

QIA10
  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species Reactivity
H
Description
Overview

This product has been discontinued.



Detects both c-ErbB2/c-Neu p185 and the extracellular domain p105 cleavage fragment. Positive cell lines: A549, HT29, SK-BR-3, and SKOV-3, or breast, prostate, colon, or ovarian cancer.
Catalogue NumberQIA10
Brand Family Calbiochem®
Synonymsc-Neu ELISA Kit, ErbB2 ELISA Kit, Erythroblastosis Virus ELISA Kit, HER-2 ELISA Kit, HER2 ELISA Kit
Application Data

In a limited study (30 cancer and 30 normal sera samples), the assay recognized 17% of cancer samples as positive and 95% of normal samples as negative.
Materials Required but Not Delivered• 2-20 µl, 20-200 µl and 200-1000 µl precision pipetters with disposable tips.
• Wash bottle or multichannel dispenser for washing.
• 500 ml graduated cylinder.
• Deionized or distilled H2O.
• Spectrophotometer capable of measuring absorbance in 96-well plates using dual wavelength of 450/595 nm or 450/540nm.
References
ReferencesGauchez, A.S., et al. 2008. Anticancer Res. 28, 3067.
Hung, M.-C. and Y-K Lau, 1999. Seminars in Oncology 26(Suppl 12), 51.
Ouyang, X., et al. 1999. Lancet 353, 1591.
Ross, J.S. and J.A. Fletcher, 1999. Am. J. Clin. Pathol. 112, S53.
Ross, J.S. and J.A. Fletcher, 1998. Stem Cells 16, 413.
Disis, M.L., et al. 1997. J. Clin. Oncol. 15, 3363.
Ross, J.S., et al. 1997. Human Pathology 28, 827.
Ross, J.S., et al. 1997. Cancer 79, 2162.
Xia, W., et al. 1997. Clin. Cancer Res. 3, 3.
Cirisano, F.D. and B.Y. Karlan, 1996. J. Soc. Gynecol. Invest. 3, 99.
Menden, H., et al. 1994. Cancer Res. Clin. Oncol. 120, 378.
Kynast, B., et al. 1993. J. Cancer Res. Clin. Oncol. 119, 249.
Gusterson, B. and R. Gelber, 1992. J. Clin. Oncol. 10, 1049.
Hetzel, D.J., et al. 1992. Gynecol. Oncol. 47, 179.
Hou, L., et al. 1992. Cancer Lett. 65, 215.
Leitzel, K., et al. 1992. J. Clin. Oncol. 10, 1436.
Carney, W.P., et al. 1991. J. Tumor Marker Oncol. 6, p. 53.
Clark, G.M. and W.L. McGuire, 1991. Cancer Res. 51, 944.
Langton, B.C., et al. 1991. Cancer Res. 51, 2593.
Paterson, M.C., et al. 1991. Cancer Res. 51, 556.
Zabrecky, J.R., et al .1991. J. Biol. Chem. 266, 1716.
Berchuck, A., et al. 1990. Cancer Res. 50, 4087.
Borg, A., et. al. 1990. Cancer Res. 50, 4332.
Inglehart, J.D., et al. 1990. Cancer Res. 50, 6701.
Kern, J.A., et al. 1990. Cancer Res. 50, 5189.
Mori, S., et al. 1990. Jpn. J. Cancer Res. 81, 489.
Paik, S., et al. 1990. J. Clin. Oncol. 8, 103.
Maguire, H.C. and M.I. Greene, 1989. Seminars in Oncology 16, 148.
Ro, J., et al. 1989. Cancer Res. 49, 6941.
Slamon, D.J., et al. 1989. Science 244, 707.
Tandon, A.K., et al. 1989. J. Clin. Oncol. 7, 1120.
Wright, C., et al. 1989. Cancer Res. 49, 2087.
Yoshida, K., et al. 1989. Virchows Arch. B Cell Pathol. Mol. Pathol. 57, 285.
Fontaine, J., et al. 1988. Oncology 45, 360.
Kraus, M.H., et al., 1987. EMBO J. 6, 605.
Slamon, D., et al. 1987. Science 235, 177.
King, C.R., et al. 1985. Science 229, 974.
Shin, C., et al. 1981. Nature 290, 261.
Henry, R.J., et al. 1974. Clinical Chemistry, Harper and Row, New York.
Product Information
Form96 Tests
Format96-well plate
Kit containsPre-Coated 96-Well Plate, c-ErbB2/c-Neu Lyophilized Standard, Cell Resuspension Buffer, Antigen Extraction Agent, Sample Diluent, Biotinylated Detector Antibody, Detector Diluent, Streptavidin Peroxidase Conjugate, Conjugate Diluent, Wash Buffer, Substrate, Stop Solution, Adhesive Plate Sealers, and a user protocol.
Positive controlc-erbB2/c-neu
Biological Information
Assay range0-3 ng/ml
Assay time4 h
Sample TypeCell lysates, biological fluids
Species Reactivity
  • Human
Safety Information
R PhraseR: 23/24/25-35-36/37/38-40-41-43

Toxic by inhalation, in contact with skin and if swallowed.
Causes severe burns.
Irritating to eyes, respiratory system and skin.
Limited evidence of a carcinogenic effect.
Risk of serious damage to eyes.
May cause sensitization by skin contact.
S PhraseS: 23-26-36/37/39-45

Do not breathe fumes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Intended useThe Calbiochem® c-erbB2/c-neu Rapid Format ELISA is a non-isotopic immunoassay for the in vitro quantitation of human c-erbB2/HER-2/neu oncoprotein in cell lysates, cell culture medium, sera and plasma.
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Storage ConditionsUpon receipt, store the entire kit at -20°C until use. Do not refreeze after thawing. Lyophilized standards must remain at -20°C. All other components may be stored at 4°C. Do not expose reagents to excessive light. Allow kit reagents to warm to room temperature before use.
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Supplemental Information
Kit containsPre-Coated 96-Well Plate, c-ErbB2/c-Neu Lyophilized Standard, Cell Resuspension Buffer, Antigen Extraction Agent, Sample Diluent, Biotinylated Detector Antibody, Detector Diluent, Streptavidin Peroxidase Conjugate, Conjugate Diluent, Wash Buffer, Substrate, Stop Solution, Adhesive Plate Sealers, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
QIA10 0