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SCC232 YUMM2.1 Mouse Melanoma Cell Line

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM2 harbors the Braf V600E mutation and is homozygous negative for wt Pten and is Cdkn2+.

SCC232
≥1X10⁶ cells/vial   
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Overview

Replacement Information
Description
Catalogue NumberSCC232
DescriptionYUMM2.1 Mouse Melanoma Cell Line
OverviewSource
The YUMM2.1 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing. The YUMM2.1 cell line harbors the Braf V600E mutation and is homozygous negative for wild-type Pten, heterozygous for Cdkn2, and characterized by expression of stable beta-catenin via partial Cre/lox recombination.
Alternate Names
  • Yale University Mouse Melanoma cell line
  • Yale University Mouse Melanoma 2.1 cell line
Background InformationThe promise of immune-based therapies in cancer and increasingly successful application of these approaches have emphasized the necessity of immune-competent models to evaluate immunological responses to cancer cells. Immunocompetent genetically-engineered mouse models with distinct genetic drivers of melanoma are essential for identifying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds

YUMM2.1 cells are syngenic with the immunocompetent C57BL/6 mouse background and retain genetic markers of the Braf/Pten mouse model, characterized by activation of Braf and inactivation of Pten and Cdkn2a. The YUMM2.1 cell line harbors a stabilized beta-catenin allele from excision of exon 3, a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis, enhances metastasis, and controls tumor differentiation. Exon 3 excision is analogous to human beta-catenin stabilizing mutations found in human melanomas.

Mutation: BrafV600E/wt Pten−/− Cdkn2+/−Bcat loxex3/wt. Partial Bcat STA/wt recombination​. Male.

References:
1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.
References
Product Information
Quality LevelMQ100
Applications
ApplicationThe YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM2 harbors the Braf V600E mutation and is homozygous negative for wt Pten and is Cdkn2+.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC232 04065269371182